AM39036RP-N Interleukin-10 / IL10 antibody

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100 Tests / €550.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Interleukin-10 / IL10 BN-10

Product Description for Interleukin-10 / IL10

Mouse anti Human Interleukin-10 / IL10 BN-10.
Presentation: PE
Product is tested for Frozen Sections, Flow Cytometry.

Properties for Interleukin-10 / IL10

Product Category Primary Antibodies
Target Category
Quantity 100 Tests
Synonyms CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF
Presentation PE
Reactivity Hu
Applications C, F
Clonality Monoclonal
Clone BN-10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P22301
GeneID:
3586
Isotype control SM10R (for use in human samples)
Add. information 1. Conjugates with brighter fluorochromes, like PE and APC, will have a greater separation than those with dyes like FITC. When populations overlap, the percentage of positive cells using a selected marker can be affected by the choice of fluorescent label.
2. Use of monoclonal antibodies in patient treatment can interfere with antigen target recognition by this reagent. This should be taken into account when samples are analyzed from patients treated in this fashion.
3. Reagent data performance is based on EDTA-treated blood. Reagent performance can be affected by the use of other anticoagulants.
Application Flow cytometry: 10 µl of antibody solution for 106 leucocytes.
The clone is also suitable for IHC (frozen tissue).
Please note:
The level of most of the cytokines produced by immune unstimulated cells is too low to be detected by flow cytometry analysis. (19)
After stimulation the level of cytokines is rising and depending on the way of stimulation, the cell population, the secretion inhibitor that is used and several other factors several cytokines are upregulated and in detectable concentrations present.
Therefore, a method comprising cell stimulation, fixation and permeabilization should be used to make detection of the intracellularly expressed cytokines possible.
Fluorochrome-labelled antibodies are effectively formulated for direct immunofluorescent staining of human cells.
Background The immune system reacts to a pathogen by activation of balanced network of the humoral and cellular immune responses. Subsequently the activated condition of the immune system will, after the elimination of the pathogen, be down-regulated to a balanced situation again. Control of the immune response requires efficient communication between the different cells involved in this response. This interaction is provided by cell/cell contact and by a complex array of mediators. Among these mediators cytokines, soluble factors produced by these cells, play an important role. Cytokines can act on other cells locally or distantly, but can be even auto regulating. Cytokines can behave stimulatory or inhibitory, or can even perform both activities, depending on the (pre)activation stage of the target cell. (3, 4)
Interleukin 10 (IL-10) belongs to the group of cytokines produced by TH2 cells and monocytes. It plays a role in B cell proliferation and antibody responses.
Concentration AM39036FC-N anti-IL-10 FITC: 100 Tests / ml
AM39036RP-N anti-IL-10 PE: 100 Tests / ml
General Readings
  1. Moore JS, Zaki MH. Clinical cytokine network cytometry. Clin Lab Med. 2001 Dec;21(4):795-809. PubMed PMID: 11770288.
  2. Santiago MA, Luca PM, Bertho AL, Azeredo-Coutinho RB, Coutinho SG. Detection of intracytoplasmic cytokines by flow cytometry. Mem Inst Oswaldo Cruz. 2000 May-Jun;95(3):401-2. PubMed PMID: 10800199.
  3. Haddad JJ. Cytokines and related receptor-mediated signaling pathways. Biochem Biophys Res Commun. 2002 Oct 4;297(4):700-13. PubMed PMID: 12359210.
  4. Townsend MJ, McKenzie AN. Unravelling the net? Cytokines and diseases. J Cell Sci 2000 Oct; 113 (Pt 20): 3549-50.
  5. Prussin C. Cytokine flow cytometry: understanding cytokine biology at the single-cell level. J Clin Immunol. 1997 May;17(3):195-204. PubMed PMID: 9168399.
  6. Pala P, Hussell T, Openshaw PJ. Flow cytometric measurement of intracellular cytokines. J Immunol Methods 2000 Sep 21; 243 (1-2): 107-24.
  7. Pala P, Hussell T, Openshaw PJ. Flow cytometric measurement of intracellular cytokines. Immunol Methods 2000 Sep 21; 243(1-2): 107-24.
  8. Romagnani S. T-cell subsets (Th1 versus Th2). Ann Allergy Asthma Immunol. 2000 Jul;85(1):9-18; quiz 18, 21. PubMed PMID: 10923599.
  9. Del Prete G. The concept of type-1 and type-2 helper T cells and their cytokines in humans. Int Rev Immunol. 1998;16(3-4):427-55. PubMed PMID: 9505198.
  10. Infante-Duarte C, Kamradt T. Th1/Th2 balance in infection. Springer Semin Immunopathol 1999; 21(3):317-38.
  11. Klein SA, Dobmeyer JM, Dobmeyer TS, Pape M, Ottmann OG, Helm EB, et al. Demonstration of the Th1 to Th2 cytokine shift during the course of HIV-1 infection using cytoplasmic cytokine detection on single cell level by flow cytometry. AIDS. 1997 Jul 15;11(9):1111-8. PubMed PMID: 9233457.
  12. Bleesing JJ, Fleisher TA. Cell function-based flow cytometry. Semin Hematol. 2001 Apr;38(2):169-78. PubMed PMID: 11309698.
  13. Le Moine A, Goldman M, Abramowicz D. Multiple pathways to allograft rejection. Transplantation. 2002 May 15;73(9):1373-81. PubMed PMID: 12023610.
  14. Zhai Y, Ghobrial RM, Busuttil RW, Kupiec-Weglinski JW. Th1 and Th2 cytokines in organ transplantation: paradigm lost? Crit Rev Immunol. 1999;19(2):155-72. PubMed PMID: 10352902.
  15. Girolomoni G, Sebastiani S, Albanesi C, Cavani A. T-cell subpopulations in the development of atopic and contact allergy. Curr Opin Immunol. 2001 Dec;13(6):733-7. PubMed PMID: 11677098.
  16. Kapsenberg ML, Hilkens CM, Jansen HM, Bos JD, Snijders A, Wierenga EA. Production and modulation of T-cell cytokines in atopic allergy. Int Arch Allergy Immunol. 1996 Jun;110(2):107-13. PubMed PMID: 8645987.
  17. Sousa AE, Chaves AF, Doroana M, Antunes F, Victorino RM. Kinetics of the changes of lymphocyte subsets defined by cytokine production at single cell level during highly active antiretroviral therapy for HIV-1 infection. J Immunol. 1999 Mar 15;162(6):3718-26. PubMed PMID: 10092835.
  18. Betts MR, Ambrozak DR, Douek DC, Bonhoeffer S, Brenchley JM, Casazza JP, et al. Analysis of total human immunodeficiency virus (HIV)-specific CD4(+) and CD8(+) T-cell responses: relationship to viral load in untreated HIV infection. J Virol. 2001 Dec;75(24):11983-91. PubMed PMID: 11711588. (Free PMC Article available, 5 images available)
  19. Rostaing L, Tkaczuk J, Durand M, Peres C, Durand D, de Préval C, et al. Kinetics of intracytoplasmic Th1 and Th2 cytokine production assessed by flow cytometry following in vitro activation of peripheral blood mononuclear cells. Cytometry. 1999 Apr 1;35(4):318-28. PubMed PMID: 10213197.
Storage Store the antibody undiluted at 2-8°C.
Fluorochrome labelled product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Cat. No. /Label EX-max (nm) / EM-max (nm):
AM39036FC-N / FITC 488 / 519
AM39036RP-N  / PE   488, 532 / 578
PE
Purification:
Affinity chromatography
Buffer System:
0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2% BSA, 0.09% sodium azide
State:
Liquid purified Ig fraction
Specificity
Specificity:
The antibody detects Interleukin 10, which is 18 kDa in size and belongs to the group of cytokines produced by activated human TH2 cells, monocytes and macrophages.
Other species not tested.
Gene ID 3586

Accessory Products

Proteins and/or Positive Controls

Proteins for Interleukin-10 / IL10 (24 products)

Catalog No. Species Pres. Purity   Source  

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €210.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €139.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 98 %
Preparation: .
Purity Detail: Purity is >98%, as determined by Coomassie stained SDS-PAGE.
E. coli
10 µg / €147.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 95 %
Preparation: .
Purity Detail: >95%, as determined by Coomassie stained SDS-PAGE.
HEK293 cells
10 µg / €164.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 (His-tagged)

Interleukin-10 / IL10 Human Purified >95.0% by SDS-PAGE E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10 (His-tagged)

Interleukin-10 / IL10 Human Purified >95.0% by SDS-PAGE E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10 (His-tagged)

Interleukin-10 / IL10 Human Purified >95.0% by SDS-PAGE E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10 (19-178, His-tag)

Interleukin-10 / IL10 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €540.00
  OriGene Technologies GmbH

Interleukin-10 / IL10 (19-178, His-tag)

Interleukin-10 / IL10 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €240.00
  OriGene Technologies GmbH

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 98 % pure by SDS-PAGE gel and HPLC analyses E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 98 % pure by SDS-PAGE gel and HPLC analyses E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10

Precursor- Protein structure and amino acid sequence Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity > 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
CHO cells
  OriGene Technologies GmbH

Interleukin-10 / IL10

Precursor- Protein structure and amino acid sequence Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity > 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
CHO cells
  OriGene Technologies GmbH

Interleukin-10 / IL10

Precursor- Protein structure and amino acid sequence Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity > 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
CHO cells
  OriGene Technologies GmbH

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human
  Abnova Taiwan Corp.

Interleukin-10 / IL10

SDS-Page: IL10 Protein [NBC1-22604] - Interleukin-10, 20.94 kDa (181 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified
  Novus Biologicals Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified
  Novus Biologicals Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified
  Novus Biologicals Inc.

Positive controls for Interleukin-10 / IL10 (1 products)

Catalog No. Species Pres. Purity   Source  

IL10

Western Blot: IL10 Lysate [NBL1-11895] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for IL10
  Novus Biologicals Inc.
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