PP1115P1 Interleukin-10 / IL10 antibody

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50 µg / €230.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Rat Interleukin-10 / IL10

PP1115P1

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Product Description for Interleukin-10 / IL10

Rabbit anti Rat Interleukin-10 / IL10.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Functional assay, Western blot / Immunoblot.

Properties for Interleukin-10 / IL10

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF
Presentation Aff - Purified
Reactivity Rt
Applications E, FN, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P29456
Immunogen:
Highly pure (>98%) E.coli derived recombinant Rat IL-10 (Cat.-No PA041).
GeneID:
25325
Add. information Centrifuge vial prior to opening.
Application Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-10 (30.00 ng/ml), a concentration of 0.45-0.80 µg/ml of this antibody is required.
Indirect ELISA: To detect Rat IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-10.
Sandwich ELISA: To detect Rat IL-10 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-10 (Cat.-No PP1115B1 or PP1115B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-10.
Western Blot: To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.
General Readings
  1. Schif-Zuck S, Wildbaum G, Karin N. Coadministration of plasmid DNA constructs encoding an encephalitogenic determinant and IL-10 elicits regulatory T cell-mediated protective immunity in the central nervous system. J Immunol. 2006 Dec 1;177(11):8241-7. PubMed PMID: 17114502.
  2. Wildbaum G, Netzer N, Karin N. Tr1 cell-dependent active tolerance blunts the pathogenic effects of determinant spreading. J Clin Invest. 2002 Sep;110(5):701-10. PubMed PMID: 12208871. (Free PMC Article available, 6 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography employing immobilized Rat IL-10 matrix.
Buffer System:
PBS, pH 7.2 without preservatives.
State:
Lyophilized (sterile filtered) purified Ig fraction.
Aff - Purified
Reconstitution:
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Specificity
Specificity:
Recognizes Rat IL10. Other species not tested.
Gene ID 25325

Accessory Products

Proteins and/or Positive Controls

Proteins for Interleukin-10 / IL10 (5 products)

Catalog No. Species Pres. Purity   Source  

Interleukin-10 / IL10

Interleukin-10 / IL10 Rat > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €139.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Rat Purified > 98 % pure by SDS-PAGE and HPLC analyses. E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10

Interleukin-10 / IL10 Rat Purified > 98 % pure by SDS-PAGE and HPLC analyses. E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10

Interleukin-10 / IL10 Rat
  Abnova Taiwan Corp.

Interleukin-10 / IL10

Interleukin-10 / IL10 Rat
  Abnova Taiwan Corp.
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