TA319291 Interleukin-10 / IL10 antibody

Rabbit polyclonal anti-IL-10 antibody

See related secondary antibodies

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1 ml / €1,341.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human Interleukin-10 / IL10

Product Description for Interleukin-10 / IL10

Rabbit anti Human Interleukin-10 / IL10.
Presentation: Serum
Product is tested for Frozen Sections, Enzyme Immunoassay, Functional assay, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Interleukin-10 / IL10

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF
Presentation Serum
Reactivity Hu
Applications C, E, FN, IP, R, WB
Clonality Polyclonal
Host Rabbit
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P22301
Immunogen:
The whole rabbit serum was prepared by repeated immunizations with recombinant Human IL-10 produced in E.coli.
GeneID:
3586
Application Neutralization.
Immunoblot: Using HRP Goat-anti-Rabbit IgG [H&L] (R1364HRP) and TMB as a substrate. A working dilution range of 1/400 to 1/800 is suggested for this application to detect IL-10 from supernatants or lysates of 2 x 10e6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS.
Immunohistochemistry: A dilution range of 1:400 is suggested for use in using either paraffin fixation or cryofixation.
Immunoprecipitation: A dilution range of 1/400 to 1/800 is suggested for this immunoassay. For Immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.
ELISA: Using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (R1364HRP) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1/400 to 1/1,200 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.
Radioimmunoassay: A dilution of 1/4,000 is suggested.
Background Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.
Concentration 80 mg/ml (by Refractometry)
General Readings
  1. Yan XT, Zhuang M, Oakes JE, Lausch RN. Autocrine action of IL-10 suppresses proinflammatory mediators and inflammation in the HSV-1-infected cornea. J Leukoc Biol. 2001 Jan;69(1):149-57. PubMed PMID: 11200059.
  2. Barbara G, Xing Z, Hogaboam CM, Gauldie J, Collins SM. Interleukin 10 gene transfer prevents experimental colitis in rats. Gut. 2000 Mar;46(3):344-9. PubMed PMID: 10673295. (Free PMC Article available, 6 images available)
Storage Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
None
State:
Liquid (sterile filtered) Serum
Serum
Specificity
Specificity:
The antibody recognizes hrIL-10. By ELISA this antibody shows less than 1% cross-reactivity with murine IL-10. Direct ELISA results in no cross-reactivity against other cytokines.
This antiserum has been heated to 56°C for 30 minutes. The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts).
In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. The antibody will not neutralize the biological activity of murine IL-10.
Endotoxin Content: < 10 pg/µl by LAL method.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for Interleukin-10 / IL10 (24 products)

Catalog No. Species Pres. Purity   Source  

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €210.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €139.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 98 %
Preparation: .
Purity Detail: Purity is >98%, as determined by Coomassie stained SDS-PAGE.
E. coli
10 µg / €147.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 95 %
Preparation: .
Purity Detail: >95%, as determined by Coomassie stained SDS-PAGE.
HEK293 cells
10 µg / €164.00
  OriGene Technologies, Inc.

Interleukin-10 / IL10 (His-tagged)

Interleukin-10 / IL10 Human Purified >95.0% by SDS-PAGE E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10 (His-tagged)

Interleukin-10 / IL10 Human Purified >95.0% by SDS-PAGE E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10 (His-tagged)

Interleukin-10 / IL10 Human Purified >95.0% by SDS-PAGE E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10 (19-178, His-tag)

Interleukin-10 / IL10 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €540.00
  OriGene Technologies GmbH

Interleukin-10 / IL10 (19-178, His-tag)

Interleukin-10 / IL10 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €240.00
  OriGene Technologies GmbH

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 98 % pure by SDS-PAGE gel and HPLC analyses E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10

Interleukin-10 / IL10 Human > 98 % pure by SDS-PAGE gel and HPLC analyses E. coli
  OriGene Technologies GmbH

Interleukin-10 / IL10

Precursor- Protein structure and amino acid sequence Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity > 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
CHO cells
  OriGene Technologies GmbH

Interleukin-10 / IL10

Precursor- Protein structure and amino acid sequence Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity > 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
CHO cells
  OriGene Technologies GmbH

Interleukin-10 / IL10

Precursor- Protein structure and amino acid sequence Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity > 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
CHO cells
  OriGene Technologies GmbH

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human
  Abnova Taiwan Corp.

Interleukin-10 / IL10

SDS-Page: IL10 Protein [NBC1-22604] - Interleukin-10, 20.94 kDa (181 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified
  Novus Biologicals Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified
  Novus Biologicals Inc.

Interleukin-10 / IL10

Interleukin-10 / IL10 Human Purified
  Novus Biologicals Inc.

Positive controls for Interleukin-10 / IL10 (1 products)

Catalog No. Species Pres. Purity   Source  

IL10

Western Blot: IL10 Lysate [NBL1-11895] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for IL10
  Novus Biologicals Inc.
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