TA302515 Interleukin-12 beta / IL12B antibody

Goat Polyclonal Antibody against IL12B / IL12p40

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0.1 mg / €360.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Human Interleukin-12 beta / IL12B

Product Description for Interleukin-12 beta / IL12B

Goat anti Human Interleukin-12 beta / IL12B.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Interleukin-12 beta / IL12B

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2
Presentation Aff - Purified
Reactivity Hu
Applications E, P, WB
Clonality Polyclonal
Host Goat
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P29460
Immunogen:
Peptide with sequence from the internal region of the protein sequence according to NP_002178.2.
AA Sequence:
C-QGKSKREKKDRVFTD
GeneID:
3593
Application Peptide ELISA: Detection Limit: 1/16000.
Western Blot: 0.1-0.3 µg/ml. Approx 37kDa band observed in Human Liver lysates (calculated MW of 37.2kDa according to NP_002178.2).
Immunohistochemistry on Paraffin Embedded Sections: 4-6 µg/ml.
In paraffin embedded Human Colon shows cytoplasm staining of individual cells between columns.
In paraffin embedded Human Kidney shows cytoplasm staining of epithelial cells of some DCT.
Background Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10; homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.
Concentration 0.5 mg/ml
General Readings
  1. Sheth K, De A, Nolan B, Friel J, Duffy A, Ricciardi R, et al. Heat shock protein 27 inhibits apoptosis in human neutrophils. J Surg Res. 2001 Jul;99(1):129-33. PubMed PMID: 11421614.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Buffer System:
Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
State:
Liquid purified IgG fraction.
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognizes Interleukin-12B (IL12B).

Accessory Products

Proteins and/or Positive Controls

Proteins for Interleukin-12 beta / IL12B (17 products)

Catalog No. Species Pres. Purity   Source  

Interleukin-12 (p80)

Interleukin-12 Human > 95 % pure by SDS-PAGE gel and HPLC analyses High-5 Insect cells
  OriGene Technologies GmbH

Interleukin-12 p80

Interleukin-12 Human > 95 % pure by SDS-PAGE gel and HPLC analyses High-5 Insect cells
  OriGene Technologies GmbH

Interleukin-12 beta / IL12B

Interleukin-12 beta / IL12B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B (full length)

Interleukin-12 beta / IL12B Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B

Interleukin-12 beta / IL12B Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
CHO cells
10 µg / €239.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B

Interleukin-12 beta / IL12B Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
10 µg / €139.00
  OriGene Technologies, Inc.

Interleukin-12 beta / IL12B (His-tagged)

Interleukin-12 beta / IL12B Human Purified >95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Interleukin-12 beta / IL12B (His-tagged)

Interleukin-12 beta / IL12B Human Purified >95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Interleukin-12 beta / IL12B (His-tagged)

Interleukin-12 beta / IL12B Human Purified >95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Interleukin-12 beta / IL12B (23-328, His-tag)

Interleukin-12 beta / IL12B Human Purified > 90 % by SDS - PAGE
0.25 mg / €940.00
  OriGene Technologies GmbH

Interleukin-12 beta / IL12B (23-328, His-tag)

Interleukin-12 beta / IL12B Human Purified > 90 % by SDS - PAGE
50 µg / €370.00
  OriGene Technologies GmbH

Interleukin-12 beta / IL12B

Interleukin-12 beta / IL12B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B

Interleukin-12 beta / IL12B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B

Interleukin-12 beta / IL12B Human
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B

Interleukin-12 beta / IL12B Human
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B

Interleukin-12 beta / IL12B Human
  Abnova Taiwan Corp.

Interleukin-12 beta / IL12B

Interleukin-12 beta / IL12B Human
  Abnova Taiwan Corp.

Positive controls for Interleukin-12 beta / IL12B (2 products)

Catalog No. Species Pres. Purity   Source  

IL12B Lysate

Western Blot: IL12B Lysate [NBL1-11901] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for IL12B
  Novus Biologicals Inc.

IL12B overexpression lysate

IL12B overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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