PP1035B2 Interleukin-15 / IL15 antibody

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50 µg / €340.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human Interleukin-15 / IL15

PP1035B2

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  • PP1035B2

Product Description for Interleukin-15 / IL15

Rabbit anti Human Interleukin-15 / IL15.
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Interleukin-15 / IL15

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms IL-15
Presentation Biotin
Reactivity Hu
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P40933
Immunogen:
Highly pure (> 98%) E.Coli derived recombinant Human IL-15 (Cat.-No PA088).
GeneID:
3600
Add. information Centrifuge vial prior to opening!
Application Direct ELISA: To detect hIL-15 by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-15. 
Sandwich ELISA: To detect hIL-15 by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 μg/ml of this antibody is required. This Biotinylated polyclonal antibody, in conjunction with purified Anti-Human IL-15 (Cat.-No PP1035P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-15. 
Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle.
IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotin
Purification:
Affinity Chromatography
Buffer System:
PBS, pH 7.2 without preservatives.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Specificity
Specificity:
This antibody reacts with Human Interleukin-15. Other species not tested.
Gene ID 3600

Accessory Products

Proteins and/or Positive Controls

Proteins for Interleukin-15 / IL15 (21 products)

Catalog No. Species Pres. Purity   Source  

Interleukin-15 / IL15

Interleukin-15 / IL15 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 (transcript variant 3)

Interleukin-15 / IL15 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €139.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 (transcript variant 3)

Interleukin-15 / IL15 Human > 98 %
Preparation: .
Purity Detail: Purity is >98%, as determined by Coomassie stained SDS-PAGE.
E. coli
10 µg / €164.00
  OriGene Technologies, Inc.

Interleukin-15 / IL15 (49-162, His-tag)

Interleukin-15 / IL15 Human Purified > 95 % by SDS PAGE E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

Interleukin-15 / IL15 (49-162, His-tag)

Interleukin-15 / IL15 Human Purified > 95 % by SDS PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

Interleukin-15 / IL15

Interleukin-15 / IL15 Human > 98 % > 98% pure by SDS-PAGE and HPLC analyses E. coli
  Acris Antibodies GmbH

Interleukin-15 / IL15

Interleukin-15 / IL15 Human > 98 % > 98% pure by SDS-PAGE and HPLC analyses E. coli
  Acris Antibodies GmbH

Interleukin-15 / IL15

Precursor- Protein structure and amino acid sequence Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  Acris Antibodies GmbH

Interleukin-15 / IL15

Precursor- Protein structure and amino acid sequence Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  Acris Antibodies GmbH

Interleukin-15 / IL15

Precursor- Protein structure and amino acid sequence Human Purified > 98 % Purified by proprietary chromatographic techniques, sterile filtered, purity greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  Acris Antibodies GmbH

Interleukin-15 / IL15

Interleukin-15 / IL15 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Interleukin-15 / IL15

Interleukin-15 / IL15 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Interleukin-15 / IL15

Interleukin-15 / IL15 Human Purified
  Abnova Taiwan Corp.

Interleukin-15 / IL15

Interleukin-15 / IL15 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Interleukin-15 / IL15

Interleukin-15 / IL15 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Interleukin-15 / IL15

Interleukin-15 / IL15 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Interleukin-15 / IL15

Interleukin-15 / IL15 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Interleukin-15 / IL15

Interleukin-15 / IL15 Human
  Abnova Taiwan Corp.

Interleukin-15 / IL15

Interleukin-15 / IL15 Human
  Abnova Taiwan Corp.

Interleukin-15 / IL15

SDS-Page: IL15 Protein [NBC1-18480] - Interleukin-15, 13.9kDa (123aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Interleukin-15 / IL15

Interleukin-15 / IL15 Human Purified
  Novus Biologicals Inc.

Positive controls for Interleukin-15 / IL15 (2 products)

Catalog No. Species Pres. Purity   Source  

IL15 293T Cell Transient Overexpression Lysate(Denatured)

IL15 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

IL15 Lysate

Western Blot: IL15 Lysate [NBL1-11907] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for IL15
  Novus Biologicals Inc.
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