AP02489PU-N IRS1 pSer639 antibody

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0.1 mg / €320.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat IRS1

AP02489PU-N

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  • AP02489PU-N

Product Description for IRS1

Rabbit anti Human, Mouse, Rat IRS1.
Properties: pSer639
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for IRS1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IRS-1, Insulin receptor substrate 1
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P35568
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 639 (P-K-Sp-V-S).
GeneID:
3667
Property pSer639
Application Western Blot: 1/500~1/10000.
Immunohistochemistry on Paraffin Sections: 1/50~1/100.
Background Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed.
Concentration 1.0 mg/ml
General Readings
  1. Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
  2. Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
  3. Steppan CM, et al. (2005) Mol Cell Biol; 25(4): 1569-1575
  4. Batty IH, et al. (2004) Biochem J; 379(Pt 3): 641-651
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer System:
BPS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State:
Liquid purified IgG fraction.
Aff - Purified
Specificity
Specificity:
This antibody detects endogenous levels of IRS-1 only when phosphorylated at Serine 639.
Species:
Human, Mouse and Rat.
Gene ID 3667

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