AP06397PU-N IRS1 antibody

See related secondary antibodies

Search for all "IRS1"

0.1 mg / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat IRS1


More Views

  • AP06397PU-N

Product Description for IRS1

Rabbit anti Human, Mouse, Rat IRS1.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for IRS1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IRS-1, Insulin receptor substrate 1
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~ 132, 180 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Synthetic peptide, corresponding to amino acids 280-320 of Human IRS-1.
Application Western blot: 1/500-1/1000.
Immunohistochemistry on paraffin sections: 1/50-1/200.
Background IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
Concentration 1.0 mg/ml
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Buffer System:
Phosphate buffered saline (PBS), pH 7.2.
0.05% sodium azide
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse and Rat.
Species reactivity (tested):
This antibody detects endogenous levels of IRS-1 protein
(region surrounding Arg301)
Gene ID 3667

Accessory Products

  • LinkedIn