AP20832PU-N IRS1 pSer639 antibody

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0.1 mg / €350.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat IRS1

Product Description for IRS1

Rabbit anti Human, Mouse, Rat IRS1.
Properties: pSer639
Presentation: Aff - Purified
Product is tested for Paraffin Sections.

Properties for IRS1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IRS-1, Insulin receptor substrate 1
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications P
Clonality Polyclonal
Host Rabbit
Molecular weight ~132, 180 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P35568
GeneID:
3667
Property pSer639
Application Immunohistochemistry on paraffin sections: 1/50 - 1/200.
Background Insulin receptor substrate-1 (IRS-1) is a 170-185 kDa substrate of the insulin receptor that undergoes phosphorylation in response to insulin, IGF-1 and IL-4. Tyrosine (Tyr) phosphorylation of IRS-1 mediates insulin-stimulated responses, while Serine (Ser)/Threonine (Thr) phosphorylation of IRS-1 can either enhance or negate insulin effects. Tyrosines 465, 612, 632, 662, 941 and 989 of IRS-1 resemble YXXM motifs that upon phosphorylation are predicted to bind SH2 domains in the p85 regulatory subunit of PI3K, resulting in activation of p110 catalytic subunit. SHP-2 binding to IRS-1 can occur upon phosphorylation at Tyr 1179 and Tyr 1229. GRB2 binding can occur upon phsophorylation at Tyr 896. Rodent Ser 99 and Thr 502 of IRS-1 are casein kinase II-dependent phosphorylation sites. There is an increase in Ser 636 phosphorylation of IRS-1 in primary skeletal muscle cells from patients with type 2 diabetes.
Concentration 1.0 mg/ml
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography (> 95% (by SDS-PAGE)
Buffer System:
Phosphate buffered saline (PBS), pH 7.2.
Preservatives:
0.05% sodium azide
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse and Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser639.
Gene ID 3667

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