TA306226 IRS1 antibody

Rabbit Polyclonal IRS-1 Antibody

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0.1 mg / €371.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse IRS1

TA306226

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  • TA306226
  • TA306226

Product Description for IRS1

Rabbit anti Human, Mouse IRS1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for IRS1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IRS-1, Insulin receptor substrate 1
Presentation Aff - Purified
Reactivity Hu, Ms
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P35568
Immunogen:
IRS-1 antibody was raised against a 16 amino acid peptide from near the center of human IRS-1.
GeneID:
3667
Add. information Blocking peptide available: AP30456CP-N
Application ELISA.
Western blot: 1 - 2 μg/ml.
Immunocytochemistry.
Background Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins (1). IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL)-4, IL-9, interferon-?, in addition to the insulin and insulin-like growth factor 1 receptors (2). The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR) (3,4). TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway (3).
General Readings
  1. Giovannone B, Scaldaferri ML, Federici M, Porzio O, Lauro D, Fusco A, et al. Insulin receptor substrate (IRS) transduction system: distinct and overlapping signaling potential. Diabetes Metab Res Rev. 2000 Nov-Dec;16(6):434-41. PubMed PMID: 11114102.
  2. Waters SB, Pessin JE. Insulin receptor substrate 1 and 2 (IRS1 and IRS2): what a tangled web we weave. Trends Cell Biol. 1996 Jan;6(1):1-4. PubMed PMID: 15157524.
  3. Ozes ON, Akca H, Mayo LD, Gustin JA, Maehama T, Dixon JE, et al. A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1. Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4640-5. Epub 2001 Apr 3. PubMed PMID: 11287630. (Free PMC Article available, 5 images available)
  4. Shamji AF, Nghiem P, Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol Cell. 2003 Aug;12(2):271-80. PubMed PMID: 14536067.
Storage Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Peptide affinity chromatography
Buffer System:
PBS containing 0.02% sodium azide
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, mouse
Specificity
Specificity:
This antibody detects IRS-1.

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