TA319246 IRS1 pSer307 antibody

Rabbit polyclonal IRS1 phospho S307 antibody

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0.1 mg / €503.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human IRS1

Product Description for IRS1

Rabbit anti Human IRS1.
Properties: pSer307
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay.

Properties for IRS1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IRS-1, Insulin receptor substrate 1
Presentation Aff - Purified
Reactivity Hu
Applications E
Clonality Polyclonal
Host Rabbit
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P35568
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 298-316 of human IRS1 protein.
AA Sequence:
Protein Sequence: Human IRS1, 1242 aa, predicted MW 131.6 kDa:
1 masppesdgf sdvrkvgylr kpksmhkrff vlraaseagg parleyyene kkwrhkssap
61 krsiplescf ninkradskn khlvalytrd ehfaiaadse aeqdswyqal lqlhnrakgh
121 hdgaaalgag ggggscsgss glgeagedls ygdvppgpaf kevwqvilkp kglgqtknli
181 giyrlcltsk tisfvklnse aaavvlqlmn irrcghsenf ffievgrsav tgpgefwmqv
241 ddsvvaqnmh etileamram sdefrprsks qsssncsnpi svplrrhhln npppsqvglt
301 rrsrtesita tspasmvggk pgsfrvrass dgegtmsrpa svdgspvsps tnrthahrhr
361 gsarlhppln hsrsipmpas rcspsatspv slsssstsgh gstsdclfpr rssasvsgsp
421 sdggfissde ygsspcdfrs sfrsvtpdsl ghtppargee elsnyicmgg kgpstltapn
481 ghyilsrggn ghrctpgtgl gtspalagde aasaadldnr frkrthsagt sptithqktp
541 sqssvasiee ytemmpaypp gggsggrlpg hrhsafvptr sypeeglemh plerrgghhr
601 pdsstlhtdd gympmspgva pvpsgrkgsg dympmspksv sapqqiinpi rrhpqrvdpn
661 gymmmspsgg cspdigggps ssssssnavp sgtsygklwt ngvgghhshv lphpkppves
721 sggkllpctg dymnmspvgd sntsspsdcy ygpedpqhkp vlsyyslprs fkhtqrpgep
781 eegarhqhlr lstssgrlly aataddssss tssdslgggy cgarlepslp hphhqvlqph
841 lprkvdtaaq tnsrlarptr lslgdpkast lprareqqqq qqpllhppep kspgeyvnie
901 fgsdqsgyls gpvafhssps vrcpsqlqpa preeetgtee ymkmdlgpgr raawqestgv
961 emgrlgpapp gaasicrptr avpssrgdym tmqmscprqs yvdtspaapv syadmrtgia
1021 aeevslprat maaassssaa sasptgpqga aelaahssll ggpqgpggms aftrvnlspn
1081 rnqsakvira dpqgcrrrhs setfsstpsa trvgntvpfg agaavggggg sssssedvkr
1141 hssasfenvw lrpgelggap kepaklcgaa gglenglnyi dldlvkdfkq cpqectpepq
1201 ppppppphqp lgsgessstr rssedlsaya sisfqkqped rq
GeneID:
3667
Property pSer307
Application This affinity purified antibody has been tested for use in ELISA (1:2,000-1:10,000) and western blot (1:250-1:1,500). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 180 kDa in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS307 of IRS1 protein.
Background Insulin Receptor Substrate 1 (IRS1) acts as a signaling molecule for IL-4, insulin and insulin-like growth factor-I (IGF-I) receptors. When phosphorylated by the insulin receptor, IRS1 binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. When bound, IRS1 typically activates phosphatidylinositol 3-kinase p85 subunit. IRS1 interacts with both the NPXY motif of tyrosine-phosphorylated IGF1R and the INSR through the PTB domain. Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Ser-307 phosphorylation is involved in the mechanism of insulin resistance in type 2 diabetes. Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM).
Concentration 0.5 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Danielsson A, Ost A, Nystrom FH, Strålfors P. Attenuation of insulin-stimulated insulin receptor substrate-1 serine 307 phosphorylation in insulin resistance of type 2 diabetes. J Biol Chem. 2005 Oct 14;280(41):34389-92. Epub 2005 Aug 29. PubMed PMID: 16129690.
  2. Andreozzi F, D'Alessandris C, Federici M, Laratta E, Del Guerra S, Del Prato S, et al. Activation of the hexosamine pathway leads to phosphorylation of insulin receptor substrate-1 on Ser307 and Ser612 and impairs the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin insulin biosynthetic pathway in RIN pancreatic beta-cells. Endocrinology. 2004 Jun;145(6):2845-57. Epub 2004 Mar 4. PubMed PMID: 15001544.
  3. Jiang G, Dallas-Yang Q, Liu F, Moller DE, Zhang BB. Salicylic acid reverses phorbol 12-myristate-13-acetate (PMA)- and tumor necrosis factor alpha (TNFalpha)-induced insulin receptor substrate 1 (IRS1) serine 307 phosphorylation and insulin resistance in human embryonic kidney 293 (HEK293) cells. J Biol Chem. 2003 Jan 3;278(1):180-6. Epub 2002 Oct 29. PubMed PMID: 12409308.
  4. Aguirre V, Werner ED, Giraud J, Lee YH, Shoelson SE, White MF. Phosphorylation of Ser307 in insulin receptor substrate-1 blocks interactions with the insulin receptor and inhibits insulin action. J Biol Chem. 2002 Jan 11;277(2):1531-7. Epub 2001 Oct 17. PubMed PMID: 11606564.
  5. Aguirre V, Uchida T, Yenush L, Davis R, White MF. The c-Jun NH(2)-terminal kinase promotes insulin resistance during association with insulin receptor substrate-1 and phosphorylation of Ser(307). J Biol Chem. 2000 Mar 24;275(12):9047-54. PubMed PMID: 10722755.
  6. Rui L, Aguirre V, Kim JK, Shulman GI, Lee A, Corbould A, et al. Insulin/IGF-1 and TNF-alpha stimulate phosphorylation of IRS-1 at inhibitory Ser307 via distinct pathways. J Clin Invest. 2001 Jan;107(2):181-9. PubMed PMID: 11160134. (Free PMC Article available, 9 images available)
Storage Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
State:
Liquid (sterile filtered) purified Ig.
Aff - Purified
Specificity
Specificity:
This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources. Reactivity of this antibody with IRS1 from other species is unknown.

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