AM33075PU-S ISM1 antibody

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25 µg / €260.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Mouse ISM1 B058E2

Product Description for ISM1

Rat anti Mouse ISM1 B058E2.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for ISM1

Product Category Primary Antibodies
Quantity 25 µg
Synonyms C20orf82, ISM, Isthmin-1
Presentation Purified
Reactivity Ms
Applications WB
Clonality Monoclonal
Clone B058E2
Host Rat
Isotype IgG2a
Molecular weight 51 kDa (Predicted)
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
A2ATD1
Immunogen:
Full length Mouse Isthmin 1 recombinant protein.
GeneID:
319909
Application Western blot: Each lot of this antibody is quality control tested. 
 
Use 1 µg/ml antibody dilution buffer for each mini-gel.
Background Isthmin is a secreted protein, and it was initially identified in the Xenopus midbrain-hindbrain organizer (MHB) or isthmus organizer, where it is highly expressed. The MHB is an important signaling center in vertebrates and regulates the polarized morphological differentiation of the adjacent tectum and cerebellum. Mouse Isthmin is a 454 amino acid protein containing a Thrombospondin Type 1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at the C-terminal. The TSR domains are highly conserved with 98% identity between mouse and human; 87-88% identity between mouse and Xenopus. The C-terminal AMOP domains are also highly conserved with 99% identity between mouse and human; 91% identity between mouse and Xenopus. Mouse isthmin nucleotide sequence is similar to the human chromosome 20 open reading frame 82 (C20orf82). The Ism1 gene is conserved in human, chimpanzee, dog, cow, chicken, and zebrafish. Isthmin has angiostatic activity in vitro and in vivo. Isthmin inhibits EC capillary network formation mainly by interfering with the early stages of in vitro angiogenesis on matrigel. Isthmin inhibits VEGF-induced EC proliferation and induces apoptosis in the presence of VEGF. Overexpression of isthmin in B16 melanoma inhibits tumor growth and tumor angiogenesis in mice. Knockdown of isthminin zebrafish embryos leads to abnormal intersegmental vessel (ISV) formation in the trunk. 
Structure: 454 aa with a predicted molecular weight of 51 kD. 
Distribution: Isthmin is highly expressed in the midbrain-hindbrain organizer.
Function: Isthmin has angiostatic activity in vitro and in vivo.
Interaction: Isthmin interacts with endothelial cells.
Concentration 0.5 mg/ml
General Readings
  1. Pera EM, Kim JI, Martinez SL, Brechner M, Li SY, Wessely O, et al. Isthmin is a novel secreted protein expressed as part of the Fgf-8 synexpression group in the Xenopus midbrain-hindbrain organizer. Mech Dev. 2002 Aug;116(1-2):169-72. PubMed PMID: 12128218.
  2. Xiang W, et al. 2009. J. Cell. Mol. Med. 15:359.
  3. Zhang Y, Chen M, Venugopal S, Zhou Y, Xiang W, Li YH, et al. Isthmin exerts pro-survival and death-promoting effect on endothelial cells through alphavbeta5 integrin depending on its physical state. Cell Death Dis. 2011 May 5;2:e153. doi: 10.1038/cddis.2011.37. PubMed PMID: 21544092. (Free PMC Article available, 8 images available)
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
PBS, pH 7.2
Preservatives:
0.05% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Mouse.
Specificity
Specificity:
This B058E2 monoclonal antibody recognizes Mouse Isthmin-1 and does not cross-react with Human.

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