NSB682 JNK1/2 antibody

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Rabbit anti Human JNK1/2

Product Description for JNK1/2

Rabbit anti Human JNK1/2.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for JNK1/2

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms JNK-1, JNK-46 PRKM8, JNK1, MAPK-8, Mitogen-activated protein kinase 8, SAPK, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1
Presentation Aff - Purified
Reactivity Hu
Applications P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide (Human) derived from the region of JNK 1 + 2 that contains threonine 183 and tyrosine 185, based on the human sequence. This region is conserved among many species including human, rat, mouse, chick, nematode (Caenorhabditis elegans), and fly (Drosophila melanogaster).
Application immunohistochemistry, Western Blot, IHC: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 49 55 kDa. Can be blocked with JNK 1 + 2 phospho T183 / Y185 and non-phospho peptides (pair)or PC12 whole cell lysate, + / - Sorbitol treatment., immunohistochemistry, Western Blot
Background JNK (c-Jun N-terminal Kinase), also referred to as Stress Activated Protein Kinase (SAPK), is one of the main mitogen-activated protein kinases (MAPKs) in mammals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2. JNK is activated by a variety of cellular signals including growth factors, inflammatory cytokines, and environmental stress. The JNK/SAPK signaling pathway involves sequential activation of MAPK kinase kinase (MEKK1), MAPK kinase 4 (MKK4) or MKK7, SAPK/JNK, and c-Jun. Full activation of JNK requires phosphorylation of a threonine and a tyrosine residue in the motif Thr-Pro-Tyr. MKK7 and MKK4 phosphorylate JNK at threonine 183 and tyrosine 185, respectively. The JNK pathway functions to modulate cell cycle, apoptotic and transcriptional responses to stress.
General Readings This product has been used in:Choudhury BK et al. In vivo role of p38 mitogen-activated protein kinase in mediating the anti-inflammatory effects of CpG oligodeoxynucleotide in murine asthma. J Immunol 169:5955-61 (2002). PubMed PMID: 12421981
Tong T et al. Involvement of the MAP kinase pathways in induction of GADD45 following UV radiation. Exp Cell Res 269:64-72 (2001). PubMed PMID: 11525640
Janulis M et al. A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity. Mol Cell Biol 21:2235-47 (2001). PubMed PMID: 11238956
General / background references:Cobb MH MAP kinase pathways. Prog Biophys Mol Biol 71:479-500 (1999). PubMed PMID: 10354710
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
Aff - Purified
JNK1&2 phosphorylated at threonine 183 and tyrosine 185., Phosphorylation site-specific selective for the dually phosphorylated form of the c-Jun N-terminal Kinase (JNK)/Stress-Activated Protein Kinase (SAPK) enzymes containing a phosphate on threonine 183 and tyrosine 185 (human JNK 1 + 2). The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated JNK1&2.

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