NB120-2875 Junctional Sarcoplasmic Reticulum antibody

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Mouse anti Guinea Pig, Rabbit Junctional Sarcoplasmic Reticulum VF1c


Product Description for Junctional Sarcoplasmic Reticulum

Mouse anti Guinea Pig, Rabbit Junctional Sarcoplasmic Reticulum VF1c.
Presentation: Ascites
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Junctional Sarcoplasmic Reticulum

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Ascites
Reactivity GP, Rb
Applications ICC/IF, WB
Clonality Monoclonal
Clone VF1c
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Rabbit skeletal muscle triads.
Application immunofluorescence, Western Blot, Immunofluorescence: use at a dilution of 1/250.Western Blot: use at a dilution of 1/1500. Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~90 kDa protein representing junctional SR in rabbit skeletal muscle extracts.Immunofluorescence staining of junctional sarcoplasmic reticulum results in increased intensity in fast (Type II) skeletal muscle over slow (Type I) skeletal muscle , immunofluorescence 1:250, Western Blot 1:1,500
Background The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR?s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR?s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR?s and RyR?s. Another skeletal muscle specific 90 kDa protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction.
General Readings This product has been used in:Campbell KP et al. Identification and characterization of the high affinity [3H]ryanodine receptor of the junctional sarcoplasmic reticulum Ca2+ release channel. J Biol Chem 262:6460-3 (1987). PubMed PMID: 2437119
General / background references:Guo W et al. Characterization and ultrastructural localization of a novel 90-kDa protein unique to skeletal muscle junctional sarcoplasmic reticulum. J Biol Chem 269:28359-65 (1994). PubMed PMID: 7961775
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer System:
0.05% Sodium Azide
This detects Junctional Sarcoplasmic Reticulum in skeletal muscle. It does not cross-react with cardiac muscle.
Guinea Pig, Rabbit

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