DM1035 Kallikrein-3 / PSA / KLK3 antibody

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0.5 mg / €420.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Kallikrein-3 / PSA / KLK3 CHYH1

Product Description for Kallikrein-3 / PSA / KLK3

Mouse anti Human Kallikrein-3 / PSA / KLK3 CHYH1.
Presentation: Purified
Product is tested for ELISA (capture), Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for Kallikrein-3 / PSA / KLK3

Product Category Primary Antibodies
Target Category
Quantity 0.5 mg
Synonyms Gamma-seminoprotein, P-30 antigen, Prostate-specific antigen, Semenogelase
Presentation Purified
Reactivity Hu
Applications C, E, E(capture), P, WB
Clonality Monoclonal
Clone CHYH1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P07288
Immunogen:
Human Prostate Specific Antigen (PSA)
GeneID:
354
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: In Sandwich ELISA this antibody can be used as a Capture antibody in combination with a Monoclonal tracer/detection antibody Clone CHYH2 (HRP conjugate Cat.-No DM1036HRP, TMB as substrate).
Recommended Capture Coating Dose: 0.5-1.0 µg/well. Under the suggested conditions approximately 1 ng/ml of PSA in human serum can be detected with an assay range of 0-200 ng/ml. 
Note: Matched pair antibodies are DM1035 (Clone CHYH1) and DM1036 (Clone CHYH2).
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections
(ABC method).
Western blot: 10 µg/ml allows visualization of 1 µg/lane Human PSA.
Background Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.
General Readings
  1. Chikkaveeraiah BV, Bhirde A, Malhotra R, Patel V, Gutkind JS, Rusling JF. Single-wall carbon nanotube forest arrays for immunoelectrochemical measurement of four protein biomarkers for prostate cancer. Anal Chem. 2009 Nov 1;81(21):9129-34. doi: 10.1021/ac9018022. PubMed PMID: 19775154. (Free PMC Article available, 4 images available)
  2. Yu X, Munge B, Patel V, Jensen G, Bhirde A, Gong JD, et al. Carbon nanotube amplification strategies for highly sensitive immunodetection of cancer biomarkers. J Am Chem Soc. 2006 Aug 30;128(34):11199-205. PubMed PMID: 16925438. (Free PMC Article available, 7 images available)
  3. Krishnan S, Mani V, Wasalathanthri D, Kumar CV, Rusling JF. Attomolar detection of a cancer biomarker protein in serum by surface plasmon resonance using superparamagnetic particle labels. Angew Chem Int Ed Engl. 2011 Feb 1;50(5):1175-8. doi: 10.1002/anie.201005607. Epub 2010 Dec 22. PubMed PMID: 21268221. (Free PMC Article available, 4 images available)
  4. Mani V, Chikkaveeraiah BV, Patel V, Gutkind JS, Rusling JF. Ultrasensitive immunosensor for cancer biomarker proteins using gold nanoparticle film electrodes and multienzyme-particle amplification. ACS Nano. 2009 Mar 24;3(3):585-94. doi: 10.1021/nn800863w. PubMed PMID: 19216571. (Free PMC Article available, 8 images available)
  5. Mani V et al. Highly efficient binding of paramagnetic beads bioconjugated with 100,000 or more antibodies to protein-coated surfaces. Anal Chem. 2012 Dec 4;84(23):10485-91.
  6. Chikkaveeraiah BV, Bhirde A, Malhotra R, Patel V, Gutkind JS, Rusling JF. Single-wall carbon nanotube forest arrays for immunoelectrochemical measurement of four protein biomarkers for prostate cancer. Anal Chem. 2009 Nov 1;81(21):9129-34. doi: 10.1021/ac9018022. PubMed PMID: 19775154. (Free PMC Article available, 4 images available)
  7. Chikkaveeraiah BV, Mani V, Patel V, Gutkind JS, Rusling JF. Microfluidic electrochemical immunoarray for ultrasensitive detection of two cancer biomarker proteins in serum. Biosens Bioelectron. 2011 Jul 15;26(11):4477-83. doi: 10.1016/j.bios.2011.05.005. Epub 2011 May 11. PubMed PMID: 21632234. (Free PMC Article available, 5 images available)
  8. Jeong-Mi Moon et al. A nanowirebased label-free immunosensor: Direct incorporation of a PSA antibody in electropolymerized polypyrrole. Biosensors and Bioelectronics. Volume 57, 15 July 2014, Pages 157–161.
Storage Upon receipt, store (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
0.01M PBS, pH 7.0 without preservatives
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore with double distilled water to a final concentration of 1.0 mg/ml.
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
DM1035 reacts with PSA. This antibody is reactive to normal prostate, benign prostate hyperplasia and prostate malignant carcinoma.
It has not reacted with other normal tissues tested.
Gene ID 354
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for Kallikrein-3 / PSA / KLK3 (14 products)

Catalog No. Species Pres. Purity   Source  

Kallikrein-3 / PSA / KLK3 (transcript variant 1)

Kallikrein-3 / PSA / KLK3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Kallikrein-3 / PSA / KLK3 (transcript variant 3)

Kallikrein-3 / PSA / KLK3 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €399.00
  OriGene Technologies, Inc.

Kallikrein-3 / PSA / KLK3

Fig.1. Western Blotting analysis of native human Prostate-specific antigen (AR26021PU-N). Reducing conditions. 
Lane 1-3: immunostaining of PSA antigen (1 mg/ml) with anti-human Prostate-specific antigen antibody (Clone A67-B/E3) dilution 1 μg/ml (1), 0.5 μg/ml (2), 0.2 μg/ml (3)). Detection by Goat anti-mouse / HRP secondary antibody. Human Purified > 95 % (by SDS-page)
0.1 mg / €300.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 (25-261, His-tag)

Kallikrein-3 / PSA / KLK3 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 (25-261, His-tag)

Kallikrein-3 / PSA / KLK3 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3

Kallikrein-3 / PSA / KLK3 Human > 96 % pure by SDS-PAGE Seminal Fluid
0.1 mg / €430.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3 (PSA-ACT Complex)

Kallikrein-3 / PSA / KLK3 Human Purified > 95 % >95% purity by SDS Page Seminal Fluid
50 µg / €980.00
  Acris Antibodies GmbH

Kallikrein-3 / PSA / KLK3

Kallikrein-3 / PSA / KLK3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Kallikrein-3 / PSA / KLK3

Kallikrein-3 / PSA / KLK3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Kallikrein-3 / PSA / KLK3

Kallikrein-3 / PSA / KLK3 Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

Kallikrein-3 / PSA / KLK3

Kallikrein-3 / PSA / KLK3 Human
0.1 mg / €450.00
  Abnova Taiwan Corp.

Kallikrein-3 / PSA / KLK3

Kallikrein-3 / PSA / KLK3 Human > 96 % Seminal Fluid
1 mg / €750.00
  BBI Solutions

Kallikrein-3 / PSA / KLK3

Kallikrein-3 / PSA / KLK3 Human > 96 % Seminal Fluid
0.1 mg / €1,140.00
  BBI Solutions

Kallikrein-3 / PSA / KLK3

Kallikrein-3 / PSA / KLK3 Human > 98 % Seminal Fluid
1 mg / €1,190.00
  HyTest Ltd.

Positive controls for Kallikrein-3 / PSA / KLK3 (2 products)

Catalog No. Species Pres. Purity   Source  

Kallikrein-3 / PSA / KLK3 Lysate(Denatured)

Kallikrein-3 / PSA / KLK3 Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.

Prostate Specific Antigen Lysate

Western Blot: Prostate Specific Antigen Lysate [NBL1-12351] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KLK3
0.1 mg / €400.00
  Novus Biologicals Inc.
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