TA326488 KCNMB2 (1-41, 218-235) antibody

Mouse Monoclonal Anti-BK Beta2 Antibody

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0.1 mg / €410.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Mouse, Rat KCNMB2 S53-32

Product Description for KCNMB2

Mouse anti Human, Mouse, Rat KCNMB2 S53-32.
Properties: (1-41, 218-235)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for KCNMB2

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms BK channel subunit beta-2, BKbeta2, Calcium-activated potassium channel subfamily M subunit beta-2, Calcium-activated potassium channel subunit beta-2, Charybdotoxin receptor subunit beta-2, Hbeta2, Hbeta3, K(VCA)beta-2, Maxi K channel subunit beta-2, Slo-beta-2
Presentation Purified
Reactivity Hu, Ms, Rt
Applications C, ICC/IF, IP, WB
Clonality Monoclonal
Clone S53-32
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Swiss Prot Num:
Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of Mouse BKBeta2 (accession number Q9CZM9).
Property 1-41, 218-235
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Western blot: 1 µg/ml
1 µg/mL was sufficient for detection of BK Beta2 in 10 µg of COS cell (lysate) transiently transfected with BKbeta3a by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Immunoprecipitation: 1.0-10 µg/ml
Immunofluorescence: 1.0-10 µg/ml
Immunocytochemistry: 0.1-1.0 µg/ml
Immunohistochemistry: 0.1-1.0 µg/ml
Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces.
There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release (3). A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia (4). This has major implications on other physiological processes in tissues other than the brain.
Concentration 1.0 mg/ml
General Readings
  1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA.
  2. www.iochannels.org
  3. 3. Wulf-Johansson H., et al. (2009) Brain Res. 1292: 1-13.
  4. Lee U.S., and Cui J. (2009) J Physiol. 587(7): 1481-489.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G Chromatography.
Buffer System:
PBS, pH 7.4 containing 50% Glycerol as stabilizer and 0.09% Sodium Azide as preservative.
Liquid purified Ig fraction.
Detects ~27 kDa.
No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4. Not yet validated for use in Mammalian brain.
Human, Mouse and Rat.

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