AM32121LE-N KDO alpha antibody

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Mouse anti KDO alpha 20


Product Description for KDO alpha

Mouse anti KDO alpha 20.
Presentation: Low Endotoxin
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for KDO alpha

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms Alpha-3-Deoxy-D-manno-Octulosonic Acid
Presentation Low Endotoxin
Applications E, WB
Clonality Monoclonal
Clone 20
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Immunoassay.
Western Blot.  
The typical starting working dilution is 1/10.

LPS is chemically composed of a lipid part, lipid A, which is responsible for the endotoxic activities of LPS, and a heteropolysaccharide which generally consits of an oligosaccharide, termed the core region, and, in some bacterial families, of the so-called O chain, composed of repeating oligosaccharide units.

Concentration > 0.2 mg/ml
General Readings
  1. Rozalski A, Brade L, Kosma P, Appelmelk BJ, Krogmann C, Brade H. Epitope specificities of murine monoclonal and rabbit polyclonal antibodies against enterobacterial lipopolysaccharides of the Re chemotype. Infect Immun. 1989 Sep;57(9):2645-52. PubMed PMID: 2474499. (Free PMC Article available)
  2. Rozalski A, Brade L, Kuhn HM, Brade H, Kosma P, Appelmelk BJ, et al. Determination of the epitope specificity of monoclonal antibodies against the inner core region of bacterial lipopolysaccharides by use of 3-deoxy-D-manno-octulosonate-containing synthetic antigens. Carbohydr Res. 1989 Oct 31;193:257-70. PubMed PMID: 2482126.
  3. van der Meer NM, Appelmelk BJ, Verweij-van Vught AM, Nimmich W, Kosma P, Thijs LG, et al. Binding studies of a monoclonal antibody specific for 3-deoxy-D-manno-octulosonic acid with a panel of Klebsiella pneumoniae lipopolysaccharides representing all of the O serotypes. Infect Immun. 1994 Mar;62(3):1052-7. PubMed PMID: 8112839. (Free PMC Article available)

Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.

0.02% Sodium Azide
Liquid Culture Medium with a Low Endotoxin level
Low Endotoxin
Species Reactivity
Species reactivity (tested):

The monoclonal antibody 20 reacts with alpha-3-deoxy-D-manno-octulosonic acid (= alpha-Kdo) of the inner core with a large panel of LPS. It does not bind to beta-Kdo or 5-deoxy-Kdo.
This antibody is a core antibody which does not require the presence of lipid A constituents for binding.

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