AM02110PU-N Keratan-Sulfate antibody

See related secondary antibodies

Search for all "Keratan-Sulfate"

0.1 mg / €510.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Keratan-Sulfate 4B3/D10

AM02110PU-N

Product Description for Keratan-Sulfate

Mouse anti Human Keratan-Sulfate 4B3/D10.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Keratan-Sulfate

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications C, E, WB
Clonality Monoclonal
Clone 4B3/D10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified Human Aggrecan
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA (less than 1 µg/ml).
Western blot (1 µg/ml).
Immunohistochemistry (1 µg/ml).
Background Aggrecan is the major proteoglycan of human articular car-tilage. The core protein is substituted by a number of keratan sulfate and chondroitin sulfate glycosaminoglycan chains. Whereas chondroitin sulfate is widely distributed throughout the body, keratan sulfate is primarily expressed in cartilage (joints, trachea, intervertebral discs) and cornea.
General Readings
  1. Fischer DC, Kolbe-Busch S, Stöcker G, Hoffmann A, Haubeck HD. Development of enzyme immunoassays specific for keratan sulphate- and core-protein-epitopes of the large aggregating proteoglycan from human articular cartilage. Eur J Clin Chem Clin Biochem. 1994 Apr;32(4):285-91. PubMed PMID: 7518698.
  2. Fischer DC, Haubeck HD, Eich K, Kolbe-Busch S, Stöcker G, Stuhlsatz HW, et al. A novel keratan sulphate domain preferentially expressed on the large aggregating proteoglycan from human articular cartilage is recognized by the monoclonal antibody 3D12/H7. Biochem J. 1996 Sep 15;318 ( Pt 3):1051-6. PubMed PMID: 8836155. (Free PMC Article available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
50 mM TRIS pH 7,4
State:
Lyophilized purified IgG fraction from Cell Culture Supernatant
Purified
Reconstitution:
Restore in aqua bidest to 1 mg/ml.
Specificity
Specificity:
This Monoclonal 4B3/D10 antibody is specific for Keratan Sulfate Glycosaminoglycan chains. Preliminary results, based on western blot analysis of keratan sulfate from different sources, indicate that mAb 4B3/10 is specific for keratan sulfate from articular cartilage and shows only minimal if any cross-reac-tivity with keratan sulfate from intervertebral disc. This is in contrast to the epitope recognized by the known mAb 5D4, that recognizes a widely distributed keratan sulfate epitope. Therefore, in addition to appliations in Immunochemistry and Immunohistochemistry, the mAb 4B3/D10 is well suited for detecting keratan sulfate fragments released during human inflammatory or degenerative joint diseases into synovial fluid and serum.
Species:
Human.

Accessory Products

  • LinkedIn