DRM004 Ki-67 (C-term) antibody

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0.5 ml / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse Ki-67 SP6

DRM004

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  • DRM004

Product Description for Ki-67

Rabbit anti Human, Mouse Ki-67 SP6.
Properties: (C-term)
Presentation: Supernatant
Product is tested for Paraffin Sections.

Properties for Ki-67

Product Category Primary Antibodies
Target Category
Quantity 0.5 ml
Synonyms Ki67 antigen, MKI67, Proliferation marker
Presentation Supernatant
Reactivity Hu, Ms
Applications P
Clonality Monoclonal
Clone SP6
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P46013
Immunogen:
A synthetic peptide from C-terminus of Human Ki-67.
GeneID:
4288
Property C-term
Application Immunohistochemistry on Paraffin Sections: Use Ki67 antibody at a 1/50-1/100 dilution in an ABC method for 30 minutes at RT.  
Pretreatment: EDTA Buffer pH8.0
Recommended Positive Control: Tonsil.
Also suitable for Western blot.
Background Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.
Product Citations
Purchased from Acris:
  1. Tüzel E. Spontaneous corpus cavernosum abscess in a healthy man using long-term androgenic anabolic steroids. World J Mens Health. 2015 Apr;33(1):36-8. doi: 10.5534/wjmh.2015.33.1.36. Epub 2015 Apr 23. PubMed PMID: 25927061. (Free PMC Article available, 4 images available)
  2. Lee W, Son Y, Jang H, Bae MJ, Kim J, Kang D, et al. Protective Effect of Administered Rolipram against Radiation-Induced Testicular Injury in Mice. World J Mens Health. 2015 Apr;33(1):20-9. doi: 10.5534/wjmh.2015.33.1.20. Epub 2015 Apr 23. PubMed PMID: 25927059. (Free PMC Article available, 10 images available)
  3. Goyvaerts L, Lemaire K, Arijs I, Auffret J, Granvik M, Van Lommel L, et al. Prolactin receptors and placental lactogen drive male mouse pancreatic islets to pregnancy-related mRNA changes. PLoS One. 2015 Mar 27;10(3):e0121868. doi: 10.1371/journal.pone.0121868. eCollection 2015. PubMed PMID: 25816302. (Free PMC Article available, 7 images available)
  4. Jeong YJ, Jung MG, Son Y, Jang JH, Lee YJ, Kim SH, et al. Coniferyl aldehyde attenuates radiation enteropathy by inhibiting cell death and promoting endothelial cell function. PLoS One. 2015 Jun 1;10(6):e0128552. doi: 10.1371/journal.pone.0128552. eCollection 2015. PubMed PMID: 26029925. (Free PMC Article available, 7 images available)
  5. Yoon JH, Seo HS, Lee J, Moon C, Lee K. Acute high-level toluene exposure decreases hippocampal neurogenesis in rats. Toxicol Ind Health. 2015 Oct 16. pii: 0748233715599087. PubMed PMID: 26475279.
Storage Store undiluted at 2-8°C. 
Shelf life: one year from despatch.
Format
Preservatives:
≤ 0.09% Sodium Azide
State:
Liquid Tissue Culture Supernatant
Supernatant
Species Reactivity
Species reactivity (tested):
Human and Mouse.
Specificity
Specificity:
Reacts with Ki-67.
Cellular Localization: Nuclear.
Gene ID 4288

Accessory Products

Proteins and/or Positive Controls

Proteins for Ki-67 (3 products)

Catalog No. Species Pres. Purity   Source  

Ki-67 (C-term DDK tag, transcript variant 1)

Ki-67 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

Ki-67

Ki-67 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Ki-67

Ki-67 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.
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