Product Description for Ki-67
Rabbit anti Human, Mouse Ki-67 SP6.
Properties: (C-term)
Presentation: Supernatant
Product is tested for Paraffin Sections.
Properties: (C-term)
Presentation: Supernatant
Product is tested for Paraffin Sections.
Properties for Ki-67
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.5 ml |
| Synonyms | Ki67 antigen, MKI67, Proliferation marker |
| Presentation | Supernatant |
| Reactivity | Hu, Ms |
| Applications | P |
| Clonality | Monoclonal |
| Clone | SP6 |
| Host | Rabbit |
| Isotype | IgG |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | C-term |
| Application | Immunohistochemistry on Paraffin Sections: Use Ki67 antibody at a 1/50-1/100 dilution in an ABC method for 30 minutes at RT. Pretreatment: EDTA Buffer pH8.0 Recommended Positive Control: Tonsil. Also suitable for Western blot. |
| Background | Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation. |
| Product Citations |
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| Storage | Store undiluted at 2-8°C. Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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| Specificity |
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| Gene ID | 4288 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Ki-67 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP710117 |
Ki-67 (C-term DDK tag, transcript variant 1) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€399.00
|
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| OriGene Technologies, Inc. | |||||||
| H00004288-Q01-10 |
Ki-67 |
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|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00004288-Q01-25 |
Ki-67 |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Ki-67 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| Acris Antibodies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| Acris Antibodies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| Acris Antibodies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| Acris Antibodies GmbH | |||||||
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