AP32851BT-N Klotho antibody

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50 µg / €340.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human Klotho

AP32851BT-N

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  • AP32851BT-N

Product Description for Klotho

Rabbit anti Human Klotho.
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Klotho

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms EC 3.2.1.31, KL, Klotho
Presentation Biotin
Reactivity Hu
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UEF7
Immunogen:
Highly pure (>98%) CHO cells derived recombinant Human Klotho (Cat.-No AR08005PU)
Application Direct ELISA: To detect Human Klotho by Direct ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Klotho. 
Sandwich ELISA: To detect Human Klotho by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with purified anti-Klotho (Cat.-No AP32851BT-S or AP32851BT-N) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Klotho. 
Western Blot: To detect Human Klotho by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Klotho is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon,. The full length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively, The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid. This circulating protein arises from the action of an as yet unidentified protease which cleaves transmembrane Klotho just above and/or within the plasma membrane. Klotho has been shown to play a key role in the signaling cascade of fibroblast growth factor-23 (FGF-23), a bone derived hormone that acts in the kidney to inhibit phosphate reabsorption and vitamin D biosynthesis. Klotho promotes FGF-23 signaling through binding to FGFRI (IIIc) which converts this canonical FGF receptor into a specific receptor for FGF-23. In the absence of Klotho the function of FGF-23 is literally abolished.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Label:
Biotin
Purification:
Affinity Chromatography
Buffer System:
PBS, pH 7.2
State:
Lyophilized (0.2µ Sterile filtered) purified protein
Reconstitution:
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Specificity
Specificity:
This antibody recognizes Human Klotho. Other species not tested.

Accessory Products

Proteins and/or Positive Controls

Proteins for Klotho (5 products)

Catalog No. Species Pres. Purity   Source  

Klotho

Klotho Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
CHO cells
20 µg / €239.00
  OriGene Technologies, Inc.

Klotho

Klotho Human > 98 % by SDS-PAGE and HPLC analyses CHO cells
  Acris Antibodies GmbH

Klotho

Klotho Human > 98 % by SDS-PAGE and HPLC analyses CHO cells
  Acris Antibodies GmbH

Klotho

Klotho Human Purified > 98 % by SDS-PAGE and HPLC CHO cells
20 µg / €330.00
  Acris Antibodies GmbH

Klotho

Klotho Human
  Abnova Taiwan Corp.

Positive controls for Klotho (2 products)

Catalog No. Species Pres. Purity   Source  

Klotho Lysate

Western Blot: Klotho Lysate [NBL1-12307] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KL
  Novus Biologicals Inc.

KL overexpression lysate

KL overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.
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