AM26416FC-N KOR-SA3544 antibody

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50 Tests / €380.00
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Quick Overview

Mouse anti Human KOR-SA3544 KOR-SA3544

Product Description for KOR-SA3544

Mouse anti Human KOR-SA3544 KOR-SA3544.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for KOR-SA3544

Product Category Primary Antibodies
Quantity 50 Tests
Presentation FITC
Reactivity Hu
Applications F
Clonality Monoclonal
Clone KOR-SA3544
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
A cell line (KOCL-22) established from bone marrow blood of patient with congenital leukemia
Isotype control SM10F (for use in human samples)
Application Flow cytometry: see protocol below.
Background

The Philadelphia chromosome (Ph1) has been implicated as the causative factor in greater than 90% of chronic myelogenous leukemia (CML), in 25–30% of adult and 2–10% of childhood acute lymphoblastic leukemia (ALL) and in rare cases of acute myelogenous leukemia (AML). The presence of the Ph in leukemic cells of ALL patients usually indicates poor prognosis and high risk. Sequential monitoring of the Ph in ALL correlates with the activity of malignant clones and predicts impending clinical relapse, and therefore is useful in guiding clinical therapeutic decisions.

Protocols

Flow cytometric analysis for whole blood cells
We usually use Falcon tubes or equivalents as reaction tubes for all step described below.
1) Add 20 μL of the FITC labeled anti-KOR-SA3544 monoclonal antibody (KOR-SA3544) into each tube.
2) Add 50 μL of whole blood into each tube. Mix well, and incubate for 30 minutes at room temperature (20~25oC).
3) Add 1 mL of washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN3] followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
4) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (for analysis on BD instruments), using the procedure recommended in the respective package inserts.
5) Add 1 mL of H2O to each tube and incubate for 10 minutes at room temperature.
6) Centrifuge at 500 x g for 1 minute at room temperature.
7) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
8) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer.

General Readings
  1. Sugita, K., et al., Leukemia 13, 779-785 (1999).
  2. Mori, T., et al., Leukemia 9, 1233-1239 (1995).
Storage Store at 2-8°C.
Shelf life: One year from despatch.
Format
Label:
FITC
Purification:
Protein A agarose
Buffer System:
PBS
Preservatives:
0.09% NaN3
Stabilizers:
1% BSA
State:
Liquid Ig fraction
Specificity
Specificity:

This monoclonal antibody KOR-SA3544 was reactive with a surface antigen expressed on Philadelphia chromosome (Ph1)-positive acute lymphoblastic leukemia (ALL) without exception (26/26 cases). The recognized antigen is a nonspecific cross-reacting antigen (NCA)-50/90 (CD66c), one of the carcinoembryonic antigen (CEA)-related glycoproteins encoded by a member of the CEA gene family.

The reactivity of this antibody has been reported as follows:

Common ALLa 5/38 (13.2%)
Early B precursor ALLb 1c/21 (4.8%)
T-ALL 0/19
B-ALL 0/6
B-CLL/HCL 0/3
Multiple myeloma 0/2
ANLL 16/56 (28.6%)
Ph1-ALL 26/26d (100%)
CML blastic crisis 0/9
T-NHL 0/5
B-NHL 0/4
Hodgkin's disease 0/1
CLL, chronic lymphocytic leukemia
HCL, hairy cell leukemia
NHL, non-Hodgkin's lymphoma
aCD10+, CD19+, HLA-DR+
bCD10-, CD19+, HLA-DR+
cOne patient with 11q23 translocation.
dEighteen patients with m-bcr, eight patients with M-bcr


 

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