TA302916 KPNA1 / Importin alpha-1 (N-term) antibody

Goat Polyclonal Antibody against KPNA1

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0.1 mg / €360.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Bovine, Canine, Human, Mouse, Porcine, Rat KPNA1 / Importin alpha-1

Product Description for KPNA1 / Importin alpha-1

Goat anti Bovine, Canine, Human, Mouse, Porcine, Rat KPNA1 / Importin alpha-1.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for KPNA1 / Importin alpha-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IPOA5, Karyopherin alpha 1, NPI-1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1-beta
Presentation Aff - Purified
Reactivity Bov, Can, Hu, Ms, Por, Rt
Applications WB
Clonality Polyclonal
Host Goat
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P52294
Immunogen:
Peptide from N Terminus of the protein sequence according to NP_002255.3
AA Sequence:
TTPGKENFRLKSY-C
GeneID:
3836
Property N-term
Application Peptide ELISA: 1/32000 (Detection Limit).
Western blot: 1-3 µg/ml. Approx 60kDa band observed in lysates of cell lines HEK293, HeLa and HEPG2 (calculated MW of 60.3kDa according to NP_002255.1).
Background Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. SRP1 (nucleoprotein interactor 1, NPI 1) is the alpha subunit of
karyopherin, which acts as the receptor for the targeted protein’s nuclear localization signal (Moroianu J. Crit 1997). Both subunits of karyopherin are required for protein docking at the NPC; the alpha subunit binds the substrate and the beta subunit acts as an adaptor (Moroianu J, et al. 1995).
Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin ß and promotes the release of both subunits from the docking site (Moroianu J. Crit 1997). The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC (Moroianu J. Crit 1997)
Concentration 0.5 mg/ml
General Readings Cortes P, Ye ZS, Baltimore D. RAG-1 interacts with the repeated amino acid motif of the human homologue of the yeast protein SRP1. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7633-7. PMID: 8052633
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH~7.3
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse, Rat, Dog Pig, Cow.
Species reactivity (tested):
Human
Specificity
Specificity:
This antibody detects Importin subunit alpha-1 at N-term.

Accessory Products

Proteins and/or Positive Controls

Proteins for KPNA1 / Importin alpha-1 (3 products)

Catalog No. Species Pres. Purity   Source  

KPNA1 / Importin alpha-1 (transcript variant 1)

KPNA1 / Importin alpha-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

KPNA1 / Importin alpha-1

KPNA1 / Importin alpha-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

KPNA1 / Importin alpha-1

KPNA1 / Importin alpha-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for KPNA1 / Importin alpha-1 (2 products)

Catalog No. Species Pres. Purity   Source  

KPNA1 293T Cell Transient Overexpression Lysate(Denatured)

KPNA1 293T Cell Transient Overexpression Lysate(Denatured) Transient overexpression cell lysate was tested with Anti-KPNA1 antibody (H00003836-B01) by Western Blots.
  Abnova Taiwan Corp.

SRP1 Lysate

Western Blot: SRP1 Lysate [NBL1-12369] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KPNA1
  Novus Biologicals Inc.
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