discontinued

AM00223PU-N Ku70/Ku80 (XRCC6/XRCC5) antibody

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0.1 mg / €450.00

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Mouse anti Human, Monkey, Xenopus Ku70/Ku80 (XRCC6/XRCC5) 162

Product Description for Ku70/Ku80 (XRCC6/XRCC5)

Mouse anti Human, Monkey, Xenopus Ku70/Ku80 (XRCC6/XRCC5) 162.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Paraffin Sections.

Properties for Ku70/Ku80 (XRCC6/XRCC5)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CTC75, CTC85, CTCBF, DNA-repair protein XRCC5, DNA-repair protein XRCC6, G22P1, Ku-70, Ku70, Ku86, TLAA, XRCC-6, XRCC6
Presentation Purified
Reactivity Hu, Mky, Xen
Applications C, F, ICC/IF, P
Clonality Monoclonal
Clone 162
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human B-cell nuclei from Plasmacytoid 2p68 Cells.
Isotype control AM03096PU-N
Application Flow Cytometry. Immunofluorescence. Immunoprecipitation (Native): use Protein A and Ab at 2 µg/mg protein lysate. Not suitable for Western Blotting. Immunohistology (Formalin/paraffin): Ab 1-2µg/ml for 30 min at RT. Recommended positive control: HeLa Cells, Tonsil.
Background The Ku autoantigen is a heterodimer of 70 kD (p70) and 80 kD (p80) proteins. The p70/p80 dimer is important for function of a 460 kD DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T-antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Concentration 0.2 mg/ml
General Readings
  1. Wang J, et. al. Molecular Biology Reports,1993, 18(1):15-28.
  2. Reeves WH, et. al. Journal of Experimental Medicine 1985 161(1):18-39.
Storage Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles! Shelf life: one year from despatch.
Format
Purification:
IgG purified from the ascites fluid by ammonium sulfate precipitation
Buffer System:
10 mM PBS with 0.09 % sodium azide as preservative and 0.2% BSA as stabilizer.
State:
Liquid
Purified
Specificity
Specificity:
Reacts with a conformational epitope present on p70/p80 dimer, which is destroyed during Western blotting. Reacts with Mouse and Rat, but verfied in immunoprecipitation only. Cellular Localization: nuclear. Mol. Wt. of Antigen: 70kD and 80kD.
Species:
Human, Monkey, and Xenopus. Does not react with with Bovine and Rabbit
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