AM00223PU-N Ku70/Ku80 (XRCC6/XRCC5) antibody

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Mouse anti Human, Monkey, Xenopus Ku70/Ku80 (XRCC6/XRCC5) 162

Product Description for Ku70/Ku80 (XRCC6/XRCC5)

Mouse anti Human, Monkey, Xenopus Ku70/Ku80 (XRCC6/XRCC5) 162.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Paraffin Sections.

Properties for Ku70/Ku80 (XRCC6/XRCC5)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CTC75, CTC85, CTCBF, DNA-repair protein XRCC5, DNA-repair protein XRCC6, G22P1, Ku-70, Ku70, Ku86, TLAA, XRCC-6, XRCC6
Presentation Purified
Reactivity Hu, Mky, Xen
Applications C, F, ICC/IF, P
Clonality Monoclonal
Clone 162
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human B-cell nuclei from Plasmacytoid 2p68 Cells.
Isotype control AM03096PU-N
Application Flow Cytometry. Immunofluorescence. Immunoprecipitation (Native): use Protein A and Ab at 2 µg/mg protein lysate. Not suitable for Western Blotting. Immunohistology (Formalin/paraffin): Ab 1-2µg/ml for 30 min at RT. Recommended positive control: HeLa Cells, Tonsil.
Background The Ku autoantigen is a heterodimer of 70 kD (p70) and 80 kD (p80) proteins. The p70/p80 dimer is important for function of a 460 kD DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T-antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Concentration 0.2 mg/ml
General Readings
  1. Wang J, et. al. Molecular Biology Reports,1993, 18(1):15-28.
  2. Reeves WH, et. al. Journal of Experimental Medicine 1985 161(1):18-39.
Storage Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles! Shelf life: one year from despatch.
IgG purified from the ascites fluid by ammonium sulfate precipitation
Buffer System:
10 mM PBS with 0.09 % sodium azide as preservative and 0.2% BSA as stabilizer.
Reacts with a conformational epitope present on p70/p80 dimer, which is destroyed during Western blotting. Reacts with Mouse and Rat, but verfied in immunoprecipitation only. Cellular Localization: nuclear. Mol. Wt. of Antigen: 70kD and 80kD.
Human, Monkey, and Xenopus. Does not react with with Bovine and Rabbit
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