R1059 L-asparaginase 1 antibody

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2 ml / €1,180.00
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Quick Overview

Rabbit anti Escherichia coli L-asparaginase 1

Product Description for L-asparaginase 1

Rabbit anti Escherichia coli L-asparaginase 1.
Presentation: Serum
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for L-asparaginase 1

Product Category Primary Antibodies
Quantity 2 ml
Synonyms L-ASNase I, L-asparaginase I, L-asparagine amidohydrolase I, ansA
Presentation Serum
Reactivity E. coli
Applications E, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Asparaginase from E. coli
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunohistochemistry, Immunoprecipitation and most immunological methods requiring high titer and specificity.
Recommended Dilutions
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Background Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity.
E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
Concentration 65.0 mg/ml (by refractometry)
Storage Store vial at 2-8°C prior to restoration.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below.
Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Prepared from monospecific antiserum by delipidation and defibrination
Buffer System:
0.02M Potassium phosphate, 0.15M Sodium Chloride, pH 7.2
Lyophilized antiserum
Restore with 2.0 ml of deionized water or equivalent.
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Asparaginase [E. coli].
Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.
Gene ID 946278

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