R1059PS L-asparaginase 1 antibody

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0.1 mg / €460.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Escherichia coli L-asparaginase 1

Product Description for L-asparaginase 1

Rabbit anti Escherichia coli L-asparaginase 1.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for L-asparaginase 1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms L-ASNase I, L-asparaginase I, L-asparagine amidohydrolase I, ansA
Presentation Purified
Reactivity E. coli
Applications E, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Asparaginase from E. coli

Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Recommended Dilutions:
ELISA: 1/5,000-1/25,000.
Western blot: 1/500-1/3,000.
Immunoprecipitation: 1/100.
This product has been assayed against 1.0 µg of asparaginase [E. coli] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-rabbit IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/12,000 of the reconstitution concentration is suggested.

Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.01 M Potassium phosphate, 0.14 M Sodium chloride, pH 7.4
0.01% Sodium azide
Lyophilized purified Ig fraction
Restore with 0,1 ml of deionized water (or equivalent).
This antibody detects asparaginase [E. coli]. Cross reactivity against asparaginase from other tissues and species may occur but have not been specifically determined.
Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified asparaginase [E. coli].

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