DM3053 Lactosylceramide antibody

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Mouse anti Human Lactosylceramide HuLy-m13


Product Description for Lactosylceramide

Mouse anti Human Lactosylceramide HuLy-m13.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for Lactosylceramide

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms CDw17
Presentation Purified
Reactivity Hu
Applications C, E, F, ICC/IF
Clonality Monoclonal
Clone HuLy-m13
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunoprecipitated complexes of human detergent PBL lysate and anti-b2m proteins.
Isotype control SM13P
Application Immunofluorescence: 5-20µg/ml. Flow cytometry: 1-5 µg/106 cells. ELISA. Immunohistochemistry: 5-10 µg/mL on frozen sections using avidin-biotin system. This antibody can be used on frozen cryostat sections. This product was not quality controlled in flow cytometry. Recommended positive control: Tonsil, Lymph Node, Granulocytes, Platelets. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.4 mg/ml
General Readings
  1. Lovering, K.E. Characterization of the T-cell surface by monoclonal antibodies. PhD thesis, University of Melbourne, 1985.
  2. Knapp, W. Leukocyte Typing IV, Oxford University Press, pp.810-811, 1989. Also see data on M119, pp.861,874,877-879,897,907,923,925.
  3. Fukoda, M.N., et.al. J. Biol. Chem. 261:2376, 1986.
  4. Nudelman
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody reacts with lactosylceramide (cer-Glc b1-¿4 Gal). This antibody reacts with all species containing lactosylceramide. This antibody reacts weakly with lactoneotetraosylceramide.

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