discontinued

DM3053 Lactosylceramide antibody

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0.2 mg / €250.00

Quick Overview

Mouse anti Human Lactosylceramide HuLy-m13

DM3053

Product Description for Lactosylceramide

Mouse anti Human Lactosylceramide HuLy-m13.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for Lactosylceramide

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms CDw17
Presentation Purified
Reactivity Hu
Applications C, E, F, ICC/IF
Clonality Monoclonal
Clone HuLy-m13
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Immunoprecipitated complexes of human detergent PBL lysate and anti-b2m proteins.
Isotype control SM13P
Application Immunofluorescence: 5-20µg/ml. Flow cytometry: 1-5 µg/106 cells. ELISA. Immunohistochemistry: 5-10 µg/mL on frozen sections using avidin-biotin system. This antibody can be used on frozen cryostat sections. This product was not quality controlled in flow cytometry. Recommended positive control: Tonsil, Lymph Node, Granulocytes, Platelets. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.4 mg/ml
General Readings
  1. Lovering, K.E. Characterization of the T-cell surface by monoclonal antibodies. PhD thesis, University of Melbourne, 1985.
  2. Knapp, W. Leukocyte Typing IV, Oxford University Press, pp.810-811, 1989. Also see data on M119, pp.861,874,877-879,897,907,923,925.
  3. Fukoda, M.N., et.al. J. Biol. Chem. 261:2376, 1986.
  4. Nudelman
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Format
State:
Purified
Specificity
Specificity:
This antibody reacts with lactosylceramide (cer-Glc b1-¿4 Gal). This antibody reacts with all species containing lactosylceramide. This antibody reacts weakly with lactoneotetraosylceramide.

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