DM3119 Laminin beta 1 chain (LAMB1) antibody

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Rat anti Hamster, Human, Mouse, Porcine Laminin beta 1 chain (LAMB1) LT3


Product Description for Laminin beta 1 chain (LAMB1)

Rat anti Hamster, Human, Mouse, Porcine Laminin beta 1 chain (LAMB1) LT3.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Laminin beta 1 chain (LAMB1)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hst, Hu, Ms, Por
Applications C, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone LT3
Host Rat
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Murine EHS Laminin preparation
Isotype control SM25P, SM25PX
Application Immunofluorescence. Immunoprecipitation1: Native and denatured; Use Protein G. Ab 2µg/mg protein lysate. Western Blotting: Ab 1-2µg/ml for 2hrs at RT. Immunohistology on Acetone-fixed frozen and Alcohol-fixed, paraffin-embedded: Use Ab at 2µg/ml for 30 min at RT. Recommended positive control: LS174T Cells, Normal Colon or Colon Carcinoma. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Ljubimov AV et. al., Exp Cell Res, 1986; 165:530-540.
  2. Ljubimov AV et. al. Lab Investigation, 1995; 72:461-473.
  3. Couchman
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This antibody reacts with laminin B chain. It stains most human and murine basement membranes, except for glomerular and arterial ones. Reacts with Human, Pig, Hamster, and Mouse. Others not tested. Cellular Localization: Basement membrane.

Mol. Wt. of Antigen: 210kD

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