discontinued

NB100-2220 LC3 antibody

See related secondary antibodies

Search for all "LC3"

Quick Overview

Rabbit anti Human, Mouse LC3

NB100-2220

More Views

  • NB100-2220

Product Description for LC3

Rabbit anti Human, Mouse LC3.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for LC3

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3A, MAP1 light chain 3-like protein 1, MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A
Presentation Aff - Purified
Reactivity Hu, Ms
Applications C, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9H492
Immunogen:
A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100).
GeneID:
84557
Application immunohistochemistry, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, By Western blot bands are seen at ~17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at ~21 kDa in mouse protein. Please note general reference (1) below for ATG5 control lysates used in our image., immunohistochemistry 1:200-1:400, immunoprecipitation 20ug/500ug of protein, Western Blot 2 ug/ml, Immunohistochemistry-Paraffin ,
Background LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
Concentration 1 mg/ml
General Readings
  1. Mizushimi, N., et al. Dissection of autophagosome formation using Apg5- deficient mouse embryonic stem cells. J. Cell Biol. 152:657-668 (2001)
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format
Purification:
Immunogen affinity purified
Buffer System:
0.1% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
This is specific for LC3.
Species:
Human, Mouse, Rat
Gene ID 81631

Accessory Products

Proteins and/or Positive Controls

Proteins for LC3 (9 products)

Catalog No. Species Pres. Purity   Source  

LC3 (full length, N-term HIS tag, transcript variant 1)

LC3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

LC3 (1-120, His-tag)

LC3 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

LC3 (1-120, His-tag)

LC3 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

LC3

LC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LC3

LC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

LC3

LC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LC3

LC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

LC3

LC3 Human Purified
  Novus Biologicals Inc.

LC3

LC3 Human Purified
  Novus Biologicals Inc.

Positive controls for LC3 (4 products)

Catalog No. Species Pres. Purity   Source  

LC3 Lysate

Western Blot: LC3 Lysate [NBL1-12843] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MAP1LC3A
  Novus Biologicals Inc.

MAP1LC3A 293T Cell Transient Overexpression Lysate(Denatured)

MAP1LC3A 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.

MAP1LC3A overexpression lysate

MAP1LC3A overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.

MAP1LC3A overexpression lysate

MAP1LC3A overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
  • LinkedIn