discontinued

NB100-2220 LC3 antibody

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0.1 ml / €410.00

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Rabbit anti Human, Mouse LC3

NB100-2220

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  • NB100-2220

Product Description for LC3

Rabbit anti Human, Mouse LC3.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for LC3

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3A, MAP1 light chain 3-like protein 1, MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A
Presentation Aff - Purified
Reactivity Hu, Ms
Applications C, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9H492
Immunogen:
A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100).
GeneID:
84557
Application immunohistochemistry, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, By Western blot bands are seen at ~17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at ~21 kDa in mouse protein. Please note general reference (1) below for ATG5 control lysates used in our image., immunohistochemistry 1:200-1:400, immunoprecipitation 20ug/500ug of protein, Western Blot 2 ug/ml, Immunohistochemistry-Paraffin ,
Background LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
Concentration 1 mg/ml
General Readings
  1. Mizushimi, N., et al. Dissection of autophagosome formation using Apg5- deficient mouse embryonic stem cells. J. Cell Biol. 152:657-668 (2001)
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format
Purification:
Immunogen affinity purified
Buffer System:
0.1% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
This is specific for LC3.
Species:
Human, Mouse, Rat
Gene ID 81631

Accessory Products

Proteins and/or Positive Controls

Proteins for LC3 (9 products)

Catalog No. Species Pres. Purity   Source  

LC3 (full length, N-term HIS tag, transcript variant 1)

LC3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

LC3 (1-120, His-tag)

LC3 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

LC3 (1-120, His-tag)

LC3 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

LC3

LC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LC3

LC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

LC3

LC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LC3

LC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

LC3

LC3 Human Purified
0.1 mg / €440.00
  Novus Biologicals Inc.

LC3

LC3 Human Purified
0.5 mg / €1,140.00
  Novus Biologicals Inc.

Positive controls for LC3 (4 products)

Catalog No. Species Pres. Purity   Source  

LC3 Lysate

Western Blot: LC3 Lysate [NBL1-12843] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MAP1LC3A
0.1 mg / €400.00
  Novus Biologicals Inc.

MAP1LC3A 293T Cell Transient Overexpression Lysate(Denatured)

MAP1LC3A 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.

MAP1LC3A overexpression lysate

MAP1LC3A overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.

MAP1LC3A overexpression lysate

MAP1LC3A overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
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