NB100-2331L LC3 antibody

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Rabbit anti Human, Mouse LC3


Product Description for LC3

Rabbit anti Human, Mouse LC3.
Presentation: HiLyte Fluor™ 488
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for LC3

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation HiLyte Fluor™ 488
Reactivity Hu, Ms
Applications C, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 50-150).
Application immunohistochemistry 1:200-1:400,,immunoprecipitation 20ug/500ug of protein,,Western Blot 2 ug/ml,,Immunohistochemistry-Paraffin ,,
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
Concentration 0.91 mg/ml
General Readings
  1. Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, et al. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 2000 Nov 1;19(21):5720-8. PubMed PMID: 11060023. (Free PMC Article available, 7 images available)
  2. Mizushima N, Yamamoto A, Hatano M, Kobayashi Y, Kabeya Y, Suzuki K, et al. Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 2001 Feb 19;152(4):657-68. PubMed PMID: 11266458. (Free PMC Article available, 9 images available)
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
HiLyte Fluor™ 488
Affinity purified
Buffer System:
Tris-glycine, 150mM NaCl
Affinity purified
Human and mouse. Other species have not been tested.

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