discontinued

NB100-2331 LC3B antibody

See related secondary antibodies

Search for all "LC3B"

0.1 ml / €400.00

Quick Overview

Rabbit anti Human, Mouse LC3B

Product Description for LC3B

Rabbit anti Human, Mouse LC3B.
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for LC3B

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B, MAP1A/MAP1B, MAP1LC3B, Map1alc3, Map1lc3, Map1lc3b, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B
Presentation Aff - Purified
Reactivity Hu, Ms
Applications IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9GZQ8
Immunogen:
A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 50-125).
GeneID:
81631
Application Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, This antibody is useful for Western blot and immunohistochemistry. By Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. There are also a few non-specific bands above ~32 kDa in mouse protein. , Immunohistochemistry-Paraffin 1:200-1:400, immunoprecipitation, Western Blot 2 ug/ml
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
Concentration 1.2 mg/ml
General Readings
  1. Kabeya, Y., et al. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. EMBO. 19(21): 5720-5728 (2000).
  2. Mizushima, N., et al. Dissection of autophagosome formation using Apg5- deficient mouse embryonic stem cells. J. Cell. Biol. 152: 657-668 (2001).
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format
Purification:
Affinity purified
Buffer System:
0.1% Sodium Azide
Purity:
Affinity purified
State:
Aff - Purified
Specificity
Specificity:
LC3
Species:
Bovine, Human, Mouse, Rat, Xenopus, Zebra Fish
Gene ID 81631

Accessory Products

Proteins and/or Positive Controls

Proteins for LC3B (12 products)

Catalog No. Species Pres. Purity   Source  

LC3B (full length, N-term HIS tag)

LC3B Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

LC3B (1-120, His-tag)

LC3B Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

LC3B (1-120, His-tag)

LC3B Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

LC3B

10ug of GTX116080-pro LC3B recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera. Purified Purified by conventional chromatography.
25 µg / €360.00
  GeneTex Inc.

LC3B

LC3B Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

LC3B

LC3B Human in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LC3B

LC3B Human in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

LC3B

LC3B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LC3B

LC3B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

LC3B

LC3B Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

LC3B

LC3B Human Purified
0.1 mg / €380.00
  Novus Biologicals Inc.

LC3B

LC3B Human Purified
0.5 mg / €870.00
  Novus Biologicals Inc.

Positive controls for LC3B (1 products)

Catalog No. Species Pres. Purity   Source  

LC3B Lysate

Western Blot: LC3B Lysate [NBL1-12844] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MAP1LC3B
0.1 mg / €400.00
  Novus Biologicals Inc.
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