NB600-1384L LC3B antibody

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Rabbit anti Human, Mouse LC3B


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  • NB600-1384L
  • NB600-1384L
  • NB600-1384L
  • NB600-1384L

Product Description for LC3B

Rabbit anti Human, Mouse LC3B.
Presentation: Serum
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for LC3B

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Serum
Reactivity Hu, Ms
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

A synthetic peptide made to the N-terminal region of the human LC3, isoform B protein.
Application immunohistochemistry 1:2000,,immunofluorescence 1:2000,,Western Blot 1:3000,,Immunocytochemistry 1:2000
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
General Readings Hiroshi Aoki, Yasunari Takada, Seiji Kondo, Raymond Sawaya, Bharat Aggarwal, and Yasuko Kondo, Evidence That Curcumin Suppresses the Growth of Malignant Gliomas In Vitro and In Vivo Through Induction of Autophagy: Role of Akt and ERK Signaling Pathway, Mol Pharmacol. 2007 Mar 29; [Epub ahead of print]
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Whole antisera
Whole antisera
This antibody reacts with the human and mouse proteins. Other species have not been tested.

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