discontinued

NB120-4900 Lck antibody

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0.05 ml / €380.00

Quick Overview

Rabbit anti Human Lck

NB120-4900

Product Description for Lck

Rabbit anti Human Lck.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Lck

Product Category Primary Antibodies
Quantity 0.05 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide (Human) derived from the region of human Lck that contains tyrosine 192 (based on Swiss Protein database, accession number P06239). The sequence is conserved in human and mouse.
Background Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of multiple sites, including the two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Phosphorylation of Lck Tyr-192 blocks high affinity binding of other proteins to the SH2 domain of Lck, thereby negatively regulating its participation in T cell antigen receptor signalling.
General Readings General / background references:Fujimaki W et al. Functional uncoupling of T-cell receptor engagement and Lck activation in anergic human thymic CD4+ T cells. J Biol Chem 276:17455-60 (2001). PubMed PMID: 11279170
Ellery JM et al. Activation of the interleukin 2 receptor: a possible role for tyrosine phosphatases. Cell Signal 12:367-73 (2000). PubMed PMID: 10889465
Gervais FG & Veillette A Reconstitution of interactions between protein-tyrosine phosphatase CD45 and tyrosine-protein kinase p56(lck) in nonlymphoid cells. J Biol Chem 272:12754-61 (1997). PubMed PMID: 9139734
Hardwick JS & Sefton BM The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505. J Biol Chem 272:25429-32 (1997). PubMed PMID: 9325251
Format
Purification:
Immunogen affinity purified
Buffer System:
Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.4
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Species:
Cross-reacts with Human.Expected to cross-react with Chicken (79% identity with immunogen), Mouse (100% identity with immunogen) due to sequence homology.Not yet tested in other species.

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