NB120-4901 Lck antibody

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Rabbit anti Human Lck


Product Description for Lck

Rabbit anti Human Lck.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Lck

Product Category Primary Antibodies
Quantity 0.05 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide (Human) derived from the region of Lck that contains tyrosine 505 (based on Swiss Protein database, accession number P06239). The sequence is conserved in human and mouse.
Background Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Tyr-505 is located near the carboxyl terminus of Lck and, when phosphorylated, associates intramolecularly with the SH2 domain in the amino-terminal half of the protein. This helps stabilise Lck in a conformation that, biologically, is relatively inactive. In the absence of phosphorylation at Tyr-505, intramolecular binding of the carboxyl terminus to the SH2 domain does not occur, and Lck exhibits increased activity in vivo.
General Readings General / background references:Fujimaki W et al. Functional uncoupling of T-cell receptor engagement and Lck activation in anergic human thymic CD4+ T cells. J Biol Chem 276:17455-60 (2001). PubMed PMID: 11279170
Ellery JM et al. Activation of the interleukin 2 receptor: a possible role for tyrosine phosphatases. Cell Signal 12:367-73 (2000). PubMed PMID: 10889465
Gervais FG & Veillette A Reconstitution of interactions between protein-tyrosine phosphatase CD45 and tyrosine-protein kinase p56(lck) in nonlymphoid cells. J Biol Chem 272:12754-61 (1997). PubMed PMID: 9139734
Hardwick JS & Sefton BM The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505. J Biol Chem 272:25429-32 (1997). PubMed PMID: 9325251
Immunogen affinity purified
Buffer System:
Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.4
Immunogen affinity purified
Aff - Purified
Cross-reacts with Human.Expected to cross-react with Chicken (92% identity with immunogen), Mouse (100% identity with immunogen) due to sequence homology.Not yet tested in other species.

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