NSB1076 LIMK 1/2 antibody

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Rabbit anti Human, Mouse, Rat LIMK 1/2

Product Description for LIMK 1/2

Rabbit anti Human, Mouse, Rat LIMK 1/2.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for LIMK 1/2

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human LIMK1 that contains tyrosine 507 and threonine 508 (tyrosine 504 and threonine 505 in LIMK2). The LIMK1 sequence is conserved in mouse and rat, and the LIMK2 sequence is conserved in mouse, rat, and chicken.
Add. information Research Areas: Cancer
Application Western Blot, The antibody has been used for Western blotting, where a band is seen around 75 kDa. , Western Blot - 1:1000
Background The Lin-11, Is1-1 and Mec-3 kinases 1 and 2 (LIMK1 [72 kDa] and LIMK2 [65 kDa]) are serine/threonine kinases that are involved in actin cytoskeletal reorganization, cell growth regulation and tumor supression. LIMK1/2 are activated by various extracellular stimuli including stromal cell-derived factor-1a (SDF-1a) and insulin, through activation of small GTPases (Rho, Rac-1 and Cdc-42) and their downstream kinases, ROCK and PAK1. ROCK directly phosphorylates LIMK1 at threonine 508 and LIMK2 at threonine 505. LIMK1 plays a role in Rac-induced lamellipodia formation, whereas LIMK2 plays a role in Cdc42-induced filipodial formation and Rho-induced stress fiber formation. LIMK1/2 negatively regulate cofilin activity by phosphorylating cofilin at serine 3 and hindering it from associating with actin.
General Readings Davila, M., et al. (2003) LIM kinase 1 is essential for the invasive growth of prostate epithelial cells: implications in prostate cancer. J. Biol. Chem. 278(38):36868-36875.
Sumi, T., et al. (2002) Mitosis-dependent phosphorylation and activation of LIM-kinase 1. Biochem. Biophys. Res. Commun. 290(4):1315-1320.
Lou, Z., et al. (2001) A role for a RhoA/ROCK/LIM-kinase pathway in the regulation of cytotoxic lymphocytes. J. Immunol. 167(10):5749-5757.
Sumi, T., et al. (2001) Specific activation of LIM kinase 2 via phosphorylation of threonine 505 by ROCK, a Rho-dependent protein kinase. J. Biol. Chem. 276(1):670-676.
Ohashi, K., et al. (2000) Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop. J. Biol. Chem. 275(5):3577-3582.
Maekawa, M., et al. (1999) Signaling from Rho to the Actin Cytoskeleton through Protein Kinases ROCK and LIM-kinase. Science 285(5429):895-898.
Yang, N., et al. (1998) Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization. Nature 393(6687):809-812.
Nunoue, K., et al. (1995) LIMK-1 and LIMK-2, two members of a LIM motif-containing protein kinase family. Oncogene 11(4):701-710.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Affinity purified
Buffer System:
0.05% Sodium Azide
Affinity purified
Aff - Purified
This is specific for LIM Kinase only when phosphorylated at threonine 507/508.
Human, Mouse, Rat

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