AM33175FC-N Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) antibody

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Mouse anti Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) UCHT1, HCD14, HIB19, 2H7, HCD56


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  • AM33175FC-N

Product Description for Lineage Cocktail (CD3, CD14, CD19, CD20, CD56)

Mouse anti Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) UCHT1, HCD14, HIB19, 2H7, HCD56.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for Lineage Cocktail (CD3, CD14, CD19, CD20, CD56)

Product Category Primary Antibodies
Quantity 50 Tests
Presentation FITC
Reactivity Hu
Applications F
Clonality Monoclonal
Clone UCHT1, HCD14, HIB19, 2H7, HCD56
Host Mouse
Isotype IgG1, IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Add. information Excitation Laser: Blue Laser (488 nm)
Application Each lot of this antibody is quality control tested by Immunofluorescent staining with Flow Cytometric analysis.
For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Background CD3 is expressed on all T lymphocytes. CD14 is expressed on monocytes, macrophages, neutrophils, and eosinophils. CD19 and CD20 are expressed on B lymphocytes. CD56 is expressed on activated and resting NK lymphocytes.
General Readings
  1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey.
  2. Olweus J, BitMansour A, Warnke R, Thompson PA, Carballido J, Picker LJ, et al. Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12551-6. PubMed PMID: 9356487. (Free PMC Article available, 5 images available)
  3. Banchereau J, et al. Eds. 1995. Advances in Experimental Medicine and Biology. New York.
  4. Markowicz S, Engleman EG. Granulocyte-macrophage colony-stimulating factor promotes differentiation and survival of human peripheral blood dendritic cells in vitro. J Clin Invest. 1990 Mar;85(3):955-61. PubMed PMID: 2179270. (Free PMC Article available)
  5. Freudenthal PS, Steinman RM. The distinct surface of human blood dendritic cells, as observed after an improved isolation method. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7698-702. PubMed PMID: 2145580. (Free PMC Article available)
  6. MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN. Characterization of human blood dendritic cell subsets. Blood. 2002 Dec 15;100(13):4512-20. Epub 2002 Aug 15. PubMed PMID: 12393628.
  7. Piccioli D, Tavarini S, Borgogni E, Steri V, Nuti S, Sammicheli C, et al. Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets. Blood. 2007 Jun 15;109(12):5371-9. Epub 2007 Mar 1. PubMed PMID: 17332250.
Storage Store undiluted at 2-8°C.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
The solution is free of unconjugated FITC.
Affinity Chromatography
Buffer System:
PBS, pH 7.2
0.09% Sodium Azide
0.2% (w/v) BSA
Liquid purified IgG fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
This anti-Human Lineage Cocktail is optimized for the detection of Human lymphocytes, monocytes, eosinophils, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells (DCs), including DCs that express CD16. 
This cocktail is composed of CD3, CD14, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.

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