BP2235 Lipopolysaccharide (LPS gram negative bacteria) antibody

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1 ml / €540.00
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Goat anti Enterobacter Lipopolysaccharide


Product Description for Lipopolysaccharide

Goat anti Enterobacter Lipopolysaccharide.
Properties: (LPS gram negative bacteria)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for Lipopolysaccharide

Product Category Primary Antibodies
Quantity 1 ml
Synonyms LPS, Lipid A
Presentation Purified
Reactivity Enterobacter
Applications ICC/IF
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Whole cells prep of Lipid A from E. coli O157
Property LPS gram negative bacteria
Application Indirect Immunofluoresence: > 1/100 (against members of the Entero-bacteriaciae).
May also be used in place of neat antiserum in almost any appropriate antibody-based technique.
Also suitable for conjugation purposes.
Background Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.
Concentration 4-5 mg/ml (OD280 nm, E0.1% = 1.4)
Product Citations
Purchased from Acris:
  • Sünderhauf, A;Skibbe, K;Preisker, S;Ebbert, K;Verschoor, A;Karsten, CM;Kemper, C;Huber-Lang, M;Basic, M;Bleich, A;Büning, J;Fellermann, K;Sina, C;Derer, S. Regulation of epithelial cell expressed C3 in the intestine - Relevance for the pathophysiology of inflammatory bowel disease? Mol. Immunol. 2017, 227-238, 90. PubMed PMID: 28843904.
General Readings
  1. Gibson DL, White AP, Snyder SD, Martin S, Heiss C, Azadi P, et al. Salmonella produces an O-antigen capsule regulated by AgfD and important for environmental persistence. J Bacteriol. 2006 Nov;188(22):7722-30. PubMed PMID: 17079680. (Free PMC Article available, 6 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Sodium Sulphate Precipitation and Ion Exchange Chromatography
Buffer System:
0.01M PBS, pH 7.2
0.09% Sodium Azide
Liquid purified Ig fraction (> 95% pure)
The antibody recognizes Lipid A / Lipopolysaccharide (LPS Gram Negative Bacteria).
Cross-reactive with numerous members of the Enterobacteriaciae: Pseudomonas aeruginosa, Klebsiella pneumoniae, E. coli O157, Salmonella enteriditis, Enterobacter aerogenes, E. hermanii, Yersinia enterocolitica and Shigella sonnei.

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