discontinued

BM3103 Lipopolysaccharide (LPS gram negative bacteria, Biphospholipid A) antibody

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Mouse anti Lipopolysaccharide (LPS gram negative bacteria, Biphospholipid A) 26-5

BM3103

Product Description for Lipopolysaccharide (LPS gram negative bacteria, Biphospholipid A)

Mouse anti Lipopolysaccharide (LPS gram negative bacteria, Biphospholipid A) 26-5.
Presentation: Ascites
Product is tested for Agglutination, Enzyme Immunoassay.

Properties for Lipopolysaccharide (LPS gram negative bacteria, Biphospholipid A)

Product Category Primary Antibodies
Quantity 1 ml
Presentation Ascites
Applications AGG, E
Clonality Monoclonal
Clone 26-5
Host Mouse
Isotype IgG2b
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic Lipid A
Application Agglutination (use undiluted).
ELISA (1:10 in phosphate buffered saline containing 4% BSA and 0.05% Tween, see also protocol below).
Concentration ~0.1 mg/ml (OD280nm, E1%=14)
Protocols For ELISA prepare bacteria suspension in PBS (5x10e8 bact./ml).
Coat ELISA plate with 100 µl/well for 1 hour at 37°C, store overnight at 2-8°C and wash 5 times with tapwater with 0.05% Tween (TT).
Incubate with diluted antibody, 1 hour at 37°C.
Wash 5 times with TT.
Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody).
Add substrate solution, incubate, stop reaction and read optical density.
General Readings Erich et al., 1989, J. Immunol., 143, 4053
Storage Store the antibody at 2-8°C. Shelf life: One year from despatch.
Format
Buffer System:
PBS containing 1% BSA and 0.09% Sodium Azide as preservative
State:
Liquid diluted ascites (sterile filtered)
Ascites
Specificity
Specificity:
Reactive with the hydrophilic region of Biphospholipid A (LPS gram negative bacteria, Lipopolysaccharide) in PHA and ELISA. In PHA, clone 26-5 induced hemolysis only with SRBC coated with Lipid A and not with ReLPs.

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