AM26274LE-N Lipoteichoic Acid (LTA) antibody

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0.2 mg / €440.00
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Mouse anti Lipoteichoic Acid (LTA) 55


Product Description for Lipoteichoic Acid (LTA)

Mouse anti Lipoteichoic Acid (LTA) 55.
Presentation: Low Endotoxin
Product is tested for Western blot / Immunoblot, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Frozen Sections.

Properties for Lipoteichoic Acid (LTA)

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Low Endotoxin
Applications C, E, ICC/IF, WB
Clonality Monoclonal
Clone 55
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977 (Ref.1).
Isotype control AM08210LE-N
Application Immunohistochemistry on Frozen Sections: The typical starting working dilution is 1/50.
Flow Cytometry: 1/250 (Ref.10).
Immunoassay: For detection, 1.2 µg/ml antibody in PBS was added for 1 hr at 37°C on LTA coated plates (Ref.2,3,8).
Immunofluorescence (Ref.4,9): 60' in PBS/0.02%BSA/0.02% Saponin (Ref.4).
Western blot (Ref 5-7): A reduced or native sample treatment and run on 15% SDS-Page.
Blot was incubated o/n at 4°C with a 1/1000 dilution. The band size is ~17 kDa (Ref.6,7).
The typical starting working dilution is 1/50.
Positive Control: Culture medium of Gram-positive bacteria.
Negative Control: Culture medium of eukaryotic cells.
Background LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2.  Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.
Concentration > 0.2 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Hogg SD, Whiley RA, De Soet JJ. Occurrence of lipoteichoic acid in oral streptococci. Int J Syst Bacteriol. 1997 Jan;47(1):62-6. PubMed PMID: 8995803.
  2. van Langevelde P, van Dissel JT, Ravensbergen E, Appelmelk BJ, Schrijver IA, Groeneveld PH. Antibiotic-induced release of lipoteichoic acid and peptidoglycan from Staphylococcus aureus: quantitative measurements and biological reactivities. Antimicrob Agents Chemother. 1998 Dec;42(12):3073-8. PubMed PMID: 9835493. (Free PMC Article available, 5 images available)
  3. van Langevelde P, Ravensbergen E, Grashoff P, Beekhuizen H, Groeneveld PH, van Dissel JT. Antibiotic-induced cell wall fragments of Staphylococcus aureus increase endothelial chemokine secretion and adhesiveness for granulocytes. Antimicrob Agents Chemother. 1999 Dec;43(12):2984-9. PubMed PMID: 10582893. (Free PMC Article available, 4 images available)
  4. Triantafilou M, Manukyan M, Mackie A, Morath S, Hartung T, Heine H, et al. Lipoteichoic acid and toll-like receptor 2 internalization and targeting to the Golgi are lipid raft-dependent. J Biol Chem. 2004 Sep 24;279(39):40882-9. Epub 2004 Jul 9. PubMed PMID: 15247273.
  5. Henneke P, Morath S, Uematsu S, Weichert S, Pfitzenmaier M, Takeuchi O, et al. Role of lipoteichoic acid in the phagocyte response to group B streptococcus. J Immunol. 2005 May 15;174(10):6449-55. PubMed PMID: 15879147.
  6. Gründling A, Schneewind O. Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus aureus. Proc Natl Acad Sci U S A. 2007 May 15;104(20):8478-83. Epub 2007 May 3. PubMed PMID: 17483484. (Free PMC Article available, 4 images available)
  7. Jimenez-Dalmaroni MJ, Xiao N, Corper AL, Verdino P, Ainge GD, Larsen DS, et al. Soluble CD36 ectodomain binds negatively charged diacylglycerol ligands and acts as a co-receptor for TLR2. PLoS One. 2009 Oct 22;4(10):e7411. doi: 10.1371/journal.pone.0007411. PubMed PMID: 19847289. (Free PMC Article available, 6 images available)
  8. Yajima A, Takahashi Y, Shimazu K, Urano-Tashiro Y, Uchikawa Y, Karibe H, et al. Contribution of phosphoglucosamine mutase to the resistance of Streptococcus gordonii DL1 to polymorphonuclear leukocyte killing. FEMS Microbiol Lett. 2009 Aug;297(2):196-202. doi: 10.1111/j.1574-6968.2009.01673.x. Epub 2009 Jun 3. PubMed PMID: 19552711.
  9. Hashimoto Y, Tabuchi Y, Sakurai K, Kutsuna M, Kurokawa K, Awasaki T, et al. Identification of lipoteichoic acid as a ligand for draper in the phagocytosis of Staphylococcus aureus by Drosophila hemocytes. J Immunol. 2009 Dec 1;183(11):7451-60. doi: 10.4049/jimmunol.0901032. Epub 2009 Nov 4. PubMed PMID: 19890048.
  10. Hirose Y, Murosaki S, Fujiki T, Yamamoto Y, Yoshikai Y, Yamashita M. Lipoteichoic acids on Lactobacillus plantarum cell surfaces correlate with induction of interleukin-12p40 production. Microbiol Immunol. 2010 Mar;54(3):143-51. doi: 10.1111/j.1348-0421.2009.00189.x. PubMed PMID: 20236424.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
0.02% Sodium Azide
Liquid Culture Medium with a Low Endotoxin level
Low Endotoxin

The monoclonal antibody 55 recognizes Lipoteichoic Acid (LTA).

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