Product Description for LSP1 / WP34
Mouse anti Human LSP1 / WP34 5E3.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.
Properties for LSP1 / WP34
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 25 µg |
| Synonyms | 47 kDa actin-binding protein, 52 kDa phosphoprotein, Lymphocyte-specific antigen WP34, Lymphocyte-specific protein 1, Protein pp52 |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | ICC/IF, WB |
| Clonality | Monoclonal |
| Clone | 5E3 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | Western blot: Each lot of this antibody is quality control tested. Recommended Dilutions: Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. Immunofluorescence (Reported). |
| Background | Leukocyte-specific protein 1 (LSP1), is a calcium-dependent F-actin binding protein and a major downstream substrate of p38 mitogen-activated protein kinase as well as protein kinase C. LSP1 plays an important role in leukocyte chemotaxis. During T cell maturation, expression of LSP1 is low on immature CD4+CD8+ double positive thymocytes, but is highly expressed on mature single positive CD4 and CD8 T cells. Distribution: Localized to the cytoplasmic leaflet of the plasma membrane. Function: Contributes to lymphocyte and neutrophil activation and chemotaxis, as well as lymphocyte differentiation. Regulation: Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Modification: Phosphorylation. Interaction: F-actin, Casein kinase II, PKC, MAPKAPK2. |
| Concentration | 0.5 mg/ml |
| General Readings |
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| Storage | Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
| Format |
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| Specificity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for LSP1 / WP34 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP300712 |
LSP1 / WP34 (transcript variant 1) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| H00004046-Q01-10 |
LSP1 / WP34 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00004046-Q01-25 |
LSP1 / WP34 |
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|
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for LSP1 / WP34 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00004046-T01 |
LSP1 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
| NBL1-12732 |
LSP1 Lysate |
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![Western Blot: LSP1 Lysate [NBL1-12732] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for LSP1](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/LSP1%20Lysate-Western%20Blot-NBL1-12732-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
| LY419390 |
LSP1 overexpression lysate |
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|
0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for LSP1 / WP34 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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