AM33059PU-S LSP1 / WP34 antibody

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Mouse anti Human LSP1 / WP34 5E3

Product Description for LSP1 / WP34

Mouse anti Human LSP1 / WP34 5E3.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for LSP1 / WP34

Product Category Primary Antibodies
Target Category
Quantity 25 µg
Synonyms 47 kDa actin-binding protein, 52 kDa phosphoprotein, Lymphocyte-specific antigen WP34, Lymphocyte-specific protein 1, Protein pp52
Presentation Purified
Reactivity Hu
Applications ICC/IF, WB
Clonality Monoclonal
Clone 5E3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
HUT 102 cell lysates.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western blot: Each lot of this antibody is quality control tested.
Recommended Dilutions: Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. 
Immunofluorescence (Reported).
Background Leukocyte-specific protein 1 (LSP1), is a calcium-dependent F-actin binding protein and a major downstream substrate of p38 mitogen-activated protein kinase as well as protein kinase C.  LSP1 plays an important role in leukocyte chemotaxis.  During T cell maturation, expression of LSP1 is low on immature CD4+CD8+ double positive thymocytes, but is highly expressed on mature single positive CD4 and CD8 T cells.
Distribution: Localized to the cytoplasmic leaflet of the plasma membrane.
Function: Contributes to lymphocyte and neutrophil activation and chemotaxis, as well as lymphocyte differentiation.
Regulation: Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2.
Modification: Phosphorylation.
Interaction: F-actin, Casein kinase II, PKC, MAPKAPK2.
Concentration 0.5 mg/ml
General Readings
  1. Jongstra-Bilen J, Young AJ, Chong R, Jongstra J. Human and mouse LSP1 genes code for highly conserved phosphoproteins. J Immunol. 1990 Feb 1;144(3):1104-10. PubMed PMID: 2295815.
  2. Kadiyala RK, McIntyre BW, Krensky AM. Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen. Eur J Immunol. 1990 Nov;20(11):2417-23. PubMed PMID: 2174784.
  3. Wu Y, Zhan L, Ai Y, Hannigan M, Gaestel M, Huang CK, et al. MAPKAPK2-mediated LSP1 phosphorylation and FMLP-induced neutrophil polarization. Biochem Biophys Res Commun. 2007 Jun 22;358(1):170-5. Epub 2007 Apr 24. PubMed PMID: 17481585.
  4. Palker TJ, Fong AM, Scearce RM, Patel DD, Haynes BF. Developmental regulation of lymphocyte-specific protein 1 (LSP1) expression in thymus during human T-cell maturation. Hybridoma. 1998 Dec;17(6):497-507. PubMed PMID: 9890705.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
PBS, pH 7.2
0.09% Sodium Azide
Liquid purified IgG fraction
This 5E3 Monoclonal antibody recognizes LSP1. Other species not tested.

Accessory Products

Proteins and/or Positive Controls

Proteins for LSP1 / WP34 (3 products)

Catalog No. Species Pres. Purity   Source  

LSP1 / WP34 (transcript variant 1)

LSP1 / WP34 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

LSP1 / WP34

LSP1 / WP34 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

LSP1 / WP34

LSP1 / WP34 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for LSP1 / WP34 (3 products)

Catalog No. Species Pres. Purity   Source  

LSP1 293T Cell Transient Overexpression Lysate(Denatured)

LSP1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

LSP1 Lysate

Western Blot: LSP1 Lysate [NBL1-12732] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for LSP1
  Novus Biologicals Inc.

LSP1 overexpression lysate

LSP1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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