AM05666BT-N Ly6A/E / SCA1 antibody

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Rat anti Mouse Ly6A/E / SCA1 D7


Product Description for Ly6A/E / SCA1

Rat anti Mouse Ly6A/E / SCA1 D7.
Presentation: Biotin
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Properties for Ly6A/E / SCA1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Ly-6A.2/Ly-6E.1, Lymphocyte antigen 6A-2/6E-1, SCA-1, Stem cell antigen 1, T-cell-activating protein, TAP
Presentation Biotin
Reactivity Ms
Applications C, F, IP, WB
Clonality Monoclonal
Clone D7
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
IL-2-dependent mouse T cell line CTL-L.
Application Western Blot.
Flow Cytometry: 1/5 - 1/10.
Use 10 µl of suggested working dilution to label 1 x 10^6 cells in 100 µl.
Immunohistochemistry on frozen sections.
Background Ly6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells.
Concentration 0.5 mg/ml
General Readings
  1. Holmes C, Stanford WL. Concise review: stem cell antigen-1: expression, function, and enigma. Stem Cells. 2007 Jun;25(6):1339-47. Epub 2007 Mar 22. PubMed PMID: 17379763.
  2. Ortega G, Korty PE, Shevach EM, Malek TR. Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity. J Immunol. 1986 Nov 15;137(10):3240-6. PubMed PMID: 2430016.
  3. Malek TR, Ortega G, Chan C, Kroczek RA, Shevach EM. Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies. J Exp Med. 1986 Sep 1;164(3):709-22. PubMed PMID: 3489060. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
Liquid purified IgG
This antibody recognises members of the lymphocyte activation protein-6 (Ly6) multigene family. Specifically, it recognises Ly6A.2 and Ly6E.1. Clone D7 has been reported to induce T cell activation.
Gene ID 110454
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