NB100-64203 Ly6c antibody

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Rat anti Mouse Ly6c ER-MP20


Product Description for Ly6c

Rat anti Mouse Ly6c ER-MP20.
Presentation: Biotin
Product is tested for Flow Cytometry.

Properties for Ly6c

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Ly-6C2, Ly6C2, Lymphocyte antigen 6C2, Mouse Macrophage Marker
Presentation Biotin
Reactivity Ms
Applications F
Clonality Monoclonal
Clone ER-MP20
Host Rat
Isotype IgG2a
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Swiss Prot Num:
Balb/c macrophage precursor cell hybrids
Application flow cytometry / FACS analysis, Flow cytometry / FACS analysis, flow cytometry / FACS analysis 1:50
Background Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes. This antibody is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15674 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. This antibody also detects a wide range of endothelial cells.
Concentration 0.1 mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
protein G purified
Buffer System:
0.09% Sodium Azide
protein G purified
NB100-64203 recognizes murine Ly-6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes. Using ER-MP20, distinct mouse blood monocyte subsets can be distinguished: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state. Studies show that clone ER-MP20 can be used in conjunction with clone ER-MP12.
Gene ID 100041546

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