discontinued

NB100-65414 Ly6c antibody

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0.25 mg / €450.00

Quick Overview

Rat anti Mouse Ly6c ER-MP20

NB100-65414

Product Description for Ly6c

Rat anti Mouse Ly6c ER-MP20.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunoprecipitation, Paraffin Sections.

Properties for Ly6c

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms Ly-6C2, Ly6C2, Lymphocyte antigen 6C2, Mouse Macrophage Marker
Presentation Purified
Reactivity Ms
Applications C, F, IP, P
Clonality Monoclonal
Clone ER-MP20
Host Rat
Isotype IgG2a
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P09568
Immunogen:
Balb/c macrophage precursor cell hybrids
GeneID:
17067
Isotype control SM15P, SM15PX
Application flow cytometry / FACS analysis, Immunohistochemistry-Frozen, immunoprecipitation, This antibody is useful for Immunohistochemistry-Frozen, immunoprecipitation, flow cytometry / FACS analysis, flow cytometry / FACS analysis 1/10 - 1/50, immunoprecipitation , Immunohistochemistry-Frozen
Background Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
Concentration 1 mg/ml
General Readings
  1. Leenen PJ, Melis M, Slieker WA, Van Ewijk W. Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens. Eur J Immunol. 1990 Jan;20(1):27-34. PubMed PMID: 2407538.
  2. de Bruijn MF, van Vianen W, Ploemacher RE, Bakker-Woudenberg IA, Campbell PA, van Ewijk W, et al. Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting. J Immunol Methods. 1998 Aug 1;217(1-2):27-39. PubMed PMID: 9776572.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
protein G purified
Buffer System:
0.09% Sodium Azide
Purity:
protein G purified
State:
Purified
Specificity
Specificity:
NB100-65414 recognizes murine Ly-6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes. Using ER-MP20, distinct mouse blood monocyte subsets can be distinguished: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state. Studies show that clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow(2).
Species:
Mouse
Gene ID 100041546

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