AM26331FC-N Ly6G / GR1 Neutrophil Marker antibody

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0.1 mg / €400.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rat anti Mouse Ly6G / GR1 Neutrophil Marker NIMP-R14

Product Description for Ly6G / GR1 Neutrophil Marker

Rat anti Mouse Ly6G / GR1 Neutrophil Marker NIMP-R14.
Presentation: FITC
Product is tested for Enzyme Immunoassay, Functional assay, Paraffin Sections, Frozen Sections.

Properties for Ly6G / GR1 Neutrophil Marker

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Gr-1 Granulocyte marker
Presentation FITC
Reactivity Ms
Applications C, E, FN, P
Clonality Monoclonal
Clone NIMP-R14
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P35461
Immunogen:
Purified BALB/c mouse neutrophils
GeneID:
546644
Application Immunohistochemistry on Frozen Sections: Tissue was fixed with acetone (Ref 6). 
The typical starting working dilution is 1/50.
Immunohistochemistry on Paraffin Sections (Ref 4,5): Blocking with 20% normal rabbit serum (Ref 4). The typical starting working dilution is 1/50.
Flow Cytometry (Ref 1,3,7): 5 x 105 cells were incubated with 10 µg/ml antibody (Ref 3).
The typical starting working dilution is 1/50.
Functional assays (Ref 2,3,5,6,8): Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1mg, 6h before infection (Ref 5). 
Immunoassays (Ref 1).
Positive Control: Mouse neutrophils.
Background The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre-monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear.
Concentration 0.1 mg/ml
General Readings
  1. Lopez AF, Strath M, Sanderson CJ. Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies. Br J Haematol. 1984 Jul;57(3):489-94. PubMed PMID: 6743568.
  2. McLaren DJ, Strath M, Smithers SR. Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells. Parasite Immunol. 1987 Nov;9(6):667-82. PubMed PMID: 3124065.
  3. Van Lent PL, Blom A, Holthuysen AE, Jacobs CW, Van De Putte LB, Van Den Berg WB. Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritis in mice. J Leukoc Biol. 1997 Mar;61(3):267-78. PubMed PMID: 9060449.
  4. Lubberts E, Joosten LA, van Den Bersselaar L, Helsen MM, Bakker AC, van Meurs JB, et al. Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction. J Immunol. 1999 Oct 15;163(8):4546-56. PubMed PMID: 10510398.
  5. Tacchini-Cottier, F et al; An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major. J Immunol 2000, 165: 628.
  6. de Vries B, Köhl J, Leclercq WK, Wolfs TG, van Bijnen AA, Heeringa P, et al. Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol. 2003 Apr 1;170(7):3883-9. PubMed PMID: 12646657.
  7. Nagendra S, Schlueter AJ. Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A. 2004 Apr;58(2):195-200. PubMed PMID: 15057973.
  8. Navarini AA, Lang KS, Verschoor A, Recher M, Zinkernagel AS, Nizet V, et al. Innate immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7107-12. doi: 10.1073/pnas.0901162106. Epub 2009 Apr 7. PubMed PMID: 19351895. (Free PMC Article available, 5 images available)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Label:
FITC
Purification:
Protein G Chromatography
Buffer System:
PBS
Preservatives:
0.02% Sodium Azide
Stabilizers:
1.0% BSA
State:
Liquid 0.2 µm filtered Ig fraction
Species Reactivity
Species reactivity (tested):
Mouse
Specificity
Specificity:
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.
It has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

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