DP3500 LYVE-1 antibody

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0.1 mg / €270.00
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Rabbit anti Human LYVE-1

DP3500

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Product Description for LYVE-1

Rabbit anti Human LYVE-1.
Presentation: Purified
Product is tested for Paraffin Sections, Flow Cytometry, Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for LYVE-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1
Presentation Purified
Reactivity Hu
Applications C, E, F, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y5Y7
Immunogen:
Recombinant Human soluble LYVE-1 (24-232) produced in insect cells.
GeneID:
10894
Remarks:
The recombinant soluble LYVE-1 consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis).
Application ELISA (1-15 µg/ml).
Western blot (1-2 µg/ml).
FACS analysis (3-20 µg/ml).
Immunohistochemistry on Frozen Sections (6-30 µg/ml).
Please Note: For use on Paraffin Embedded Sections the Affinity Purified antibody (Cat.-No DP3500PS) is recommended.
Background LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosamino-glycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Protocols Double staining LYVE-1/CD31 on Humanen Cryo-Sections
- dry Cryo sections for 1h at RT
- fix sections for 10 min at –20°C in MeOH
- wash 3x3 min in PBS at RT
- block 20 min in 10% goat serum in PBS
- 1. AB over night at 4°C in 10% goat serum in PBS
[anti h -LYVE-1 10μg/ml and anti h-CD31 1:50 (Klon JC70A)]
- wash 3x3 min in PBS at RT
- 2. AB 30 min at RT in PBS
[goat anti rabbit IgG (H+L) CY3 1:500 and goat anti mouse biotin 1:500]
- wash 3x3 min in PBS at RT
- staining of nucleus 10 min at RT in Hoechst Dye 1:5000 in PBS
- wash 3x3 min in PBS at RT
- cover with DAKO Fluorescent Mountingmedium

Staining protocol for Rabbit polyclonal anti-LYVE-1 on Paraffin Sections (Human Spleen)
The specimens (maximum edge length 0.5cm) are fixed for 24 hours (or longer) in 3.7% formalin in tap water (low pH during fixation is advantageous). Embedding is done in paraffin carefully avoiding temperatures exceeding 60°C. The sections are cut and deposited on silanized slides and dried at 60°C overnight. They are then de-paraffinized and pretreated with protease XIV (Sigma No. P-5147) at 0.5mg/ml in TBS pH7.6 for 15min at room temperature. The primary polyclonal antibodies are applied at 1:100 to 1:150 in
PBS/1%BSA/0.1%NaN3 overnight.
Subsequently, the Vector anti-rabbit ABC kit for peroxidase is used according to the manufacturer´s instructions and the presence of peroxidase revealed by diaminobenzidine reaction.
Note: The antibodies have to be used at a tenfold higher concentration on paraffin sections in comparison to cryo sections. High temperature antigen retrieval does not work, but protease pretreatment is mandatory.
The protocol was established at the laboratory of:
Prof. Dr. Birte Steiniger
Institute of Anatomy and Cell Biology
Robert-Koch-Str.8
D-35037 Marburg
Germany
E-mail: steinigb@staff.uni-marburg.de
Product Citations
Purchased from Acris:
  1. Geissler HJ, Dashkevich A, Fischer UM, Fries JW, Kuhn-Régnier F, Addicks K, et al. First year changes of myocardial lymphatic endothelial markers in heart transplant recipients. Eur J Cardiothorac Surg. 2006 May;29(5):767-71. Epub 2006 Jan 24. PubMed PMID: 16439147.
  2. Kimura H, Miyashita H, Suzuki Y, Kobayashi M, Watanabe K, Sonoda H, et al. Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis. Blood. 2009 May 7;113(19):4810-8. doi: 10.1182/blood-2008-07-170316. Epub 2009 Feb 9. PubMed PMID: 19204325.
General Readings
  1. Mouta Carreira C, Nasser SM, di Tomaso E, Padera TP, Boucher Y, Tomarev SI, et al. LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis. Cancer Res. 2001 Nov 15;61(22):8079-84. PubMed PMID: 11719431.
  2. Jackson DG. The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends Cardiovasc Med. 2003 Jan;13(1):1-7. PubMed PMID: 12554094.
  3. Sleeman JP, Krishnan J, Kirkin V, Baumann P. Markers for the lymphatic endothelium: in search of the holy grail? Microsc Res Tech. 2001 Oct 15;55(2):61-9. PubMed PMID: 11596151.
  4. Mäkinen T, Veikkola T, Mustjoki S, Karpanen T, Catimel B, Nice EC, et al. Isolated lymphatic endothelial cells transduce growth, survival and migratory signals via the VEGF-C/D receptor VEGFR-3. EMBO J. 2001 Sep 3;20(17):4762-73. PubMed PMID: 11532940. (Free PMC Article available, 8 images available)
Storage The lyophilized IgG is stable at 2-8°C for one month or at -20°C for one year from despatch.
When reconstituted the antibody is stable for six weeks at 2-8°C and at -20°C for longer.
Aliquot to avoid repeated freezing and thawing.
Format
Purification:
Protein A Chromatography (+ his tag depleted)
Buffer System:
5mM PBS, pH 7.2 without preservatives or stabilizers
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
The antibody recognizes LYVE-1.
Gene ID 10894
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for LYVE-1 (10 products)

Catalog No. Species Pres. Purity   Source  

LYVE-1 (25-235, His-tag)

LYVE-1 Human Purified > 90 % by SDS-PAGE High-5 Insect cells
0.25 mg / €1,500.00
  Acris Antibodies GmbH

LYVE-1 (25-235, His-tag)

LYVE-1 Human Purified > 90 % by SDS-PAGE High-5 Insect cells
50 µg / €520.00
  Acris Antibodies GmbH

LYVE-1

SDS-PAGE analysis of recombinant human soluble LYVE-1 from insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €200.00
  Acris Antibodies GmbH

LYVE-1

SDS-PAGE analysis of recombinant human soluble LYVE-1 from insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % by SDS-PAGE and visualised by silver stain Insect cells
25 µg / €330.00
  Acris Antibodies GmbH

LYVE-1

LYVE-1 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

LYVE-1

LYVE-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

LYVE-1

LYVE-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

LYVE-1

LYVE-1 Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

LYVE-1

LYVE-1 Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

LYVE-1

SDS-Page: LYVE1 Protein [NBC1-18514] - LYVE1, 24.8 kDa (229 aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE Protein
50 µg / €620.00
  Novus Biologicals Inc.

Positive controls for LYVE-1 (2 products)

Catalog No. Species Pres. Purity   Source  

LYVE1 Lysate

Western Blot: LYVE1 Lysate [NBL1-12770] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for LYVE1
0.1 mg / €400.00
  Novus Biologicals Inc.

XLKD1 293T Cell Transient Overexpression Lysate(Denatured)

XLKD1 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.
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