DP3513 LYVE-1 antibody

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0.2 mg / €370.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Mouse LYVE-1

DP3513

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Product Description for LYVE-1

Rabbit anti Mouse LYVE-1.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry.

Properties for LYVE-1

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1
Presentation Purified
Reactivity Ms
Applications C, E, F, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8BHC0
Immunogen:
Highly pure recombinant Mouse soluble LYVE-1 (Ala24-Gly228) produced in insect cells (Cat.-No DA3524).
This recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).
GeneID:
114332
Application ELISA (1-15 µg/ml).
Western blot (1-5 µg/ml).
FACS analysis (3-10 µg/ml).
Immunofluorescence.
Immunohistochemistry on Frozen Sections
(1-5 µg/ml).
For formalin-fixed, paraffin-embedded sections use the immunogen affinity purified antibody Cat.-No DP3513P.
Background LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212- esidue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE- molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymphspecific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Product Citations
Purchased from Acris:
  1. Conrad C, Niess H, Huss R, Huber S, von Luettichau I, Nelson PJ, et al. Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo. Circulation. 2009 Jan 20;119(2):281-9. doi: 10.1161/CIRCULATIONAHA.108.793208. Epub 2008 Dec 31. PubMed PMID: 19118255.
  2. Heishi T, Hosaka T, Suzuki Y, Miyashita H, Oike Y, Takahashi T, et al. Endogenous angiogenesis inhibitor vasohibin1 exhibits broad-spectrum antilymphangiogenic activity and suppresses lymph node metastasis. Am J Pathol. 2010 Apr;176(4):1950-8. doi: 10.2353/ajpath.2010.090829. Epub 2010 Feb 4. PubMed PMID: 20133819. (Free PMC Article available, 8 images available)
  3. Rangel-Moreno J, de la Luz Garcia-Hernandez M, Ramos-Payan R, Biear J, Hernady E, Sangster MY, et al. Long-Lasting Impact of Neonatal Exposure to Total Body Gamma Radiation on Secondary Lymphoid Organ Structure and Function. Radiat Res. 2015 Oct;184(4):352-66. doi: 10.1667/RR14047.1. Epub 2015 Sep 23. PubMed PMID: 26397175. (Free PMC Article available)
General Readings
  1. Mouta Carreira C, Nasser SM, di Tomaso E, Padera TP, Boucher Y, Tomarev SI, et al. LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis. Cancer Res. 2001 Nov 15;61(22):8079-84. PubMed PMID: 11719431.
  2. Jackson DG. The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends Cardiovasc Med. 2003 Jan;13(1):1-7. PubMed PMID: 12554094.
  3. Sleeman JP, Krishnan J, Kirkin V, Baumann P. Markers for the lymphatic endothelium: in search of the holy grail? Microsc Res Tech. 2001 Oct 15;55(2):61-9. PubMed PMID: 11596151.
  4. Mäkinen T, Veikkola T, Mustjoki S, Karpanen T, Catimel B, Nice EC, et al. Isolated lymphatic endothelial cells transduce growth, survival and migratory signals via the VEGF-C/D receptor VEGFR-3. EMBO J. 2001 Sep 3;20(17):4762-73. PubMed PMID: 11532940. (Free PMC Article available, 8 images available)
Storage The lyophilized IgG is stable at 2-8°C for one month and at -20°C for longer.
When reconstituted the antibody is stable for at least six weeks at 2-8°C.
For longer store in aliquots at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography (+his tag depletetion)
Buffer System:
PBS, pH 7.2 without preservatives or stabilizers
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore in sterile water/PBS to a concentration of 0.1-1.0 mg/ml.
Specificity
Specificity:
This antibody detetcs Mouse Lyve-1. 
This antibody is not reactive with Human LYVE-1.
Gene ID 114332
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for LYVE-1 (2 products)

Catalog No. Species Pres. Purity   Source  

LYVE-1

SDS-PAGE analysis of recombinant mouse soluble LYVE-1 from insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Mouse Purified > 95 % by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €200.00
  Acris Antibodies GmbH

LYVE-1

SDS-PAGE analysis of recombinant mouse soluble LYVE-1 from insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Mouse Purified > 95 % by SDS-PAGE and visualised by silver stain Insect cells
25 µg / €330.00
  Acris Antibodies GmbH
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