DP3513P LYVE-1 antibody

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50 µg / €280.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Mouse, Rat LYVE-1

DP3513P

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  • DP3513P

Product Description for LYVE-1

Rabbit anti Mouse, Rat LYVE-1.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for LYVE-1

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1
Presentation Aff - Purified
Reactivity Ms, Rt
Applications C, E, F, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8BHC0
Immunogen:
Highly pure (> 95%) recombinant Mouse soluble LYVE-1 (Ala24-Gly228) produced in insect cells (Cat.-No DA3524).
GeneID:
114332
Remarks:
The recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).
Application ELISA: 1-15 µg/ml.
Western blot: 2-5 µg/ml.
FACS analysis: 3-10 µg/ml. 
Immunofluorescence.
Immunohistochemistry on Paraffin and Frozen Sections: 0.25-4 µg/ml.
Background LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Product Citations
Purchased from Acris:
  1. Conrad C, Niess H, Huss R, Huber S, von Luettichau I, Nelson PJ, et al. Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo. Circulation. 2009 Jan 20;119(2):281-9. doi: 10.1161/CIRCULATIONAHA.108.793208. Epub 2008 Dec 31. PubMed PMID: 19118255.
  2. Heishi T, Hosaka T, Suzuki Y, Miyashita H, Oike Y, Takahashi T, et al. Endogenous angiogenesis inhibitor vasohibin1 exhibits broad-spectrum antilymphangiogenic activity and suppresses lymph node metastasis. Am J Pathol. 2010 Apr;176(4):1950-8. doi: 10.2353/ajpath.2010.090829. Epub 2010 Feb 4. PubMed PMID: 20133819. (Free PMC Article available, 8 images available)
Originator or purchased from resellers:
  1. Nakao S, Zandi S, Faez S, Kohno R, Hafezi-Moghadam A. Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots. FASEB J. 2012 Feb;26(2):808-17. doi: 10.1096/fj.11-183897. Epub 2011 Nov 16. PubMed PMID: 22090317. (Free PMC Article available, 7 images available)
General Readings
  1. Mouta Carreira C, Nasser SM, di Tomaso E, Padera TP, Boucher Y, Tomarev SI, et al. LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis. Cancer Res. 2001 Nov 15;61(22):8079-84. PubMed PMID: 11719431.
  2. Jackson DG. The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends Cardiovasc Med. 2003 Jan;13(1):1-7. PubMed PMID: 12554094.
  3. Sleeman JP, Krishnan J, Kirkin V, Baumann P. Markers for the lymphatic endothelium: in search of the holy grail? Microsc Res Tech. 2001 Oct 15;55(2):61-9. PubMed PMID: 11596151.
  4. Mäkinen T, Veikkola T, Mustjoki S, Karpanen T, Catimel B, Nice EC, et al. Isolated lymphatic endothelial cells transduce growth, survival and migratory signals via the VEGF-C/D receptor VEGFR-3. EMBO J. 2001 Sep 3;20(17):4762-73. PubMed PMID: 11532940. (Free PMC Article available, 8 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Antigen Affinity Chromatography
Buffer System:
PBS, pH 7.4, without preservatives or stabilizers
State:
Lyophilized purified IgG fraction
Aff - Purified
Reconstitution:
Restore in distilled sterile Water to a concentration of 0.1-1.0 mg/ml.
Species Reactivity
Species reactivity (tested):
Mouse, Rat.
Specificity
Specificity:
Cross reactivity of anti-Mouse Lyve-1 with Rat tissue.
The anti-Mouse Lyve-1 polyclonal antibody Cat.-No DP3513P shows a strong cross reaction with Rat Lyve-1 protein.
Gene ID 114332
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for LYVE-1 (2 products)

Catalog No. Species Pres. Purity   Source  

LYVE-1

SDS-PAGE analysis of recombinant mouse soluble LYVE-1 from insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Mouse Purified > 95 % by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €200.00
  Acris Antibodies GmbH

LYVE-1

SDS-PAGE analysis of recombinant mouse soluble LYVE-1 from insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Mouse Purified > 95 % by SDS-PAGE and visualised by silver stain Insect cells
25 µg / €330.00
  Acris Antibodies GmbH
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