BM4008S Macrophage F4/80 antigen antibody

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Rat anti Mouse Macrophage F4/80 antigen Cl:A3-1


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Product Description for Macrophage F4/80 antigen

Rat anti Mouse Macrophage F4/80 antigen Cl:A3-1.
Presentation: Purified
Product is tested for Immunoelectron Microscopy, Frozen Sections, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Macrophage F4/80 antigen

Product Category Primary Antibodies
Target Category
Quantity 0.25 mg
Synonyms Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
Presentation Purified
Reactivity Ms
Applications C, EM, F, IP, P, R, WB
Clonality Monoclonal
Clone Cl:A3-1
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Thioglycollate stimulated peritoneal macrophages from C57/BL mice.
Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
Application RIA.
Western Blot.
Immunoelectron Microscopy. 

Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl.
Immunohistochemistry on Frozen and Paraffin Embedded and Resin Sections. This product requires pre-treatment of paraffin sections (Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours).
Background F4/80 antigen is a 160 kD glycoprotein expressed by most murine macrophages.
Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymphnode. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Concentration 1.0 mg/ml
Protocols 1. Enzyme pre-treatment using Proteinase K (Recommended for tissues fixed for 24 hours in neutral buffered formalin, NBF):
A. TE buffer (50 mM Tris base, 1 mM EDTA, pH 8.0)
Tris Base, 6.10 g
EDTA, 0.37 g
Distilled water, 1000 ml
Mix to dissolve. Adjust pH to 8.0 using concentrated HCl (10 M HCl). Store at room temperature.

B. Proteinase K stock solution (20x, 400 μg/ml in TE buffer, pH 8.0)
Proteinase K, 4 mg
TE buffer, pH 8.0, (Reagent A) 10 ml
Mix well. Store in aliquots at -20°C.

C. Proteinase K working solution (1x, 20 μg/ml in TE buffer, pH 8.0)
Proteinase K stock solution (20x), (Reagent B) 1 ml
TE Buffer, pH 8.0, (Reagent A) 19 ml
Mix well. Discard working solution after use.

1. Dewax paraffin sections and rehydrate using preferred procedure.
2. Cover sections completely with Proteinase K working solution and incubate for 3 minutes at RT.
3. Rinse sections with Phosphate Buffered Saline (PBS).
4. Proceed with serum blocking and preferred staining protocol.

2. Heat-mediated antigen retrieval using citrate buffer, pH 6.0 (Recommended for tissues fixed for 7 days or more in neutral buffered formalin, NBF):
Citrate buffer (10 mM citric acid, pH 6.0)

Citric acid (anhydrous), 1.92 g
Distilled water, 1000 ml
Mix to dissolve. Adjust pH to 6.0 with 1 M NaOH (be sure to mix well). Store this solution at RT for 3 months, or at 4°C for longer usage.

1. Dewax paraffin sections and rehydrate using preferred protocol.
2. Pre-heat sodium citrate buffer in a staining vessel to 95-100°C.
3. Immerse slides in the citrate buffer and incubate for 10 minutes at 95-100°C. Check the citrate buffer level, add more if necessary, and then incubate for a further 10 minutes at 95-100°C.
4. Allow sections to cool for 20 minutes.
5. Rinse sections with PBS.
6. Proceed with serum blocking and preferred staining protocol.
Product Citations
Purchased from Acris:
  1. Kakehashi H, Nishioku T, Tsukuba T, Kadowaki T, Nakamura S, Yamamoto K. Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E. J Immunol. 2007 Nov 1;179(9):5728-37. PubMed PMID: 17947645.
  2. Kimura H, Miyashita H, Suzuki Y, Kobayashi M, Watanabe K, Sonoda H, et al. Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis. Blood. 2009 May 7;113(19):4810-8. doi: 10.1182/blood-2008-07-170316. Epub 2009 Feb 9. PubMed PMID: 19204325.
  3. Cuzić S, Bosnar M, Kramarić MD, Ferencić Z, Marković D, Glojnarić I, et al. Claudin-3 and Clara cell 10 kDa protein as early signals of cigarette smoke-induced epithelial injury along alveolar ducts. Toxicol Pathol. 2012 Dec;40(8):1169-87. doi: 10.1177/0192623312448937. Epub 2012 Jun 1. PubMed PMID: 22659244.
Originator or purchased from resellers:
  1. Nakao S, Zandi S, Faez S, Kohno R, Hafezi-Moghadam A. Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots. FASEB J. 2012 Feb;26(2):808-17. doi: 10.1096/fj.11-183897. Epub 2011 Nov 16. PubMed PMID: 22090317. (Free PMC Article available, 7 images available)
  2. Anghelina M, Krishnan P, Moldovan L, Moldovan NI. Monocytes/macrophages cooperate with progenitor cells during neovascularization and tissue repair: conversion of cell columns into fibrovascular bundles. Am J Pathol. 2006 Feb;168(2):529-41. PubMed PMID: 16436667. (Free PMC Article available, 8 images available)
General Readings
  1. Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981 Oct;11(10):805-15. PubMed PMID: 7308288.
  2. Hume DA, Perry VH, Gordon S. The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia. Anat Rec. 1984 Nov;210(3):503-12. PubMed PMID: 6524692.
  3. Lee SH, Starkey PM, Gordon S. Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80. J Exp Med. 1985 Mar 1;161(3):475-89. PubMed PMID: 3973536. (Free PMC Article available)
  4. Gordon S, Lawson L, Rabinowitz S, Crocker PR, Morris L, Perry VH. Antigen markers of macrophage differentiation in murine tissues. Curr Top Microbiol Immunol. 1992;181:1-37. PubMed PMID: 1424778.
  5. Warschkau H, Kiderlen AF. A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes. J Immunol. 1999 Sep 15;163(6):3409-16. PubMed PMID: 10477612.
  6. Lin HH, Faunce DE, Stacey M, Terajewicz A, Nakamura T, Zhang-Hoover J, et al. The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance. J Exp Med. 2005 May 16;201(10):1615-25. Epub 2005 May 9. PubMed PMID: 15883173. (Free PMC Article available, 6 images available)
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Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4
0.09% Sodium Azide
Liquid purified IgG fraction
Species Reactivity
Species reactivity (tested):
This antibody recognizes the F4/80 antigen, a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with Human EMR1.
Clone CI:A31 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5). We recommend the use of BM4008LE for this purpose.
Gene ID 13733
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