discontinued

BM4010 Macrophage Galactose Specific Lectin (MMGL) antibody

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0.1 mg / €480.00

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Rat anti Mouse Macrophage Galactose Specific Lectin (MMGL) ER-MP23

BM4010

Product Description for Macrophage Galactose Specific Lectin (MMGL)

Rat anti Mouse Macrophage Galactose Specific Lectin (MMGL) ER-MP23.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry.

Properties for Macrophage Galactose Specific Lectin (MMGL)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Connective Tissue Macrophage / Histiocyte Marker
Presentation Purified
Reactivity Ms
Applications C, F
Clonality Monoclonal
Clone ER-MP23
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mouse macrophage cell lines
Isotype control SM15P, SM15PX
Add. information Staining of isolated cells:
The antigen is found on the surface of connective tissue macrophages and their precursor cells in bone marrow. Macrophage cell lines (J774-1.6,RAW309Cr.1, WR19M.1) corresponding to mature macrophages express high levels of the antigen.
Staining of tissues:
The antigen is abundant on mature macrophages in connective tissues as present in the skin (dermis), salivary glands, capsule of lymph nodes, thymus and various other organs. The antigen is partly coexpressed with the BM8 antigen.
ER-MP23 positive macrophages may express Ia antigens.
Application Immunohistochemistry on frozen sections (Acetone Fixation): Approximate working dilution is 1 µg/ml (1:200 of stock solution) freshly prepared.
Use mouse skin for positive control.
The antibody has been described to work in Flow cytometry.
Background MMGL is a glutaraldehyde (0.05%) resistant 38kD single chain surface glycoprotein.
Concentration 0.2 mg/ml (stock solution)
General Readings Jansen A. et al.: Immunohistochemical characterization of monocytes-macrophages and dendritic cells involved in the initiation of the insulitis and b-cell destruction in NOD mice. Diabetes 43: 667-675 (1994).
Storage Store the unopend vial at 2-8°C for up to one year and the stock solution in aliquots at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity purified IgG
Buffer System:
PBS, pH 7.2 with 10 mg/ml BSA as stabilizer and 0.01% Thimerosal as preservative
State:
Lyophilised
Purified
Reconstitution:
Restore in 0.5 ml distilled water to make stock solution.
Specificity
Specificity:
ER-MP23 detects mature macrophages in connective tissues which are situated in the vicinity of epithelia e.g. salivary glands, capsule of lymph nodes, thymus and various other organs.
The antibody blocks the mouse macrophage galactose-specific lectin (MMGL).
Species:
Mouse.

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