BM4037B Macrophages antibody

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0.1 mg / €460.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Bovine, Canine, Feline, Human, Monkey, Porcine Macrophages PM-2K

BM4037B

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  • BM4037B
  • BM4037B

Product Description for Macrophages

Mouse anti Bovine, Canine, Feline, Human, Monkey, Porcine Macrophages PM-2K.
Presentation: Purified
Product is tested for Frozen Sections.

Properties for Macrophages

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Macrophage marker
Presentation Purified
Reactivity Bov, Can, Fe, Hu, Mky, Por
Applications C
Clonality Monoclonal
Clone PM-2K
Host Mouse
Isotype IgG1
Shipping to USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Cultured human peritoneal macrophages
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunohistochemistry on frozen section: 0.2 mg/ml (1:1000).
Suggested positive control: Human tonsil.
Does not react on routinely processed paraffin sections.
Background Monoclonal antibody PM-2K, together with X-4 and X-14, forms a particular group of macrophage specific antibodies which were tested at the Vth Leukocyte Typing Workshop. The PM-2K antigen was initially suspected to function as a scavenger receptor. However, experiments with CHO cells expressing Macrophage Scavenger Receptor (MSR) AI or AII showed no reactivity with this antibody.
Format
Purification:
 
Buffer System:
Phosphate buffered saline pH 7.2 (PBS)
Preservatives:
0.05 % (v/v) Kathon CG
Stabilizers:
5 mg/ml bovine serum albumin (BSA)
State:
Lyophilized affinity purified Ig fraction
Purified
Reconstitution:
Reconstitute by adding 0.5 ml distilled water.
Species Reactivity
Species reactivity (tested):
Human, Cat, Dog, Pig, Cow, and Monkey.Negative in Rabbit, Rat, Guinea pig, Gold hamster, Goat, and Horse.
Specificity
Specificity:
This antibody detects Macrophages.
Antigen distribution: Isolated cells: Positive on >90% of alveolar macrophages, and on 10% of adherent peritoneal cells after 1 day culture. Negative on dendritic cells, on freshly isolated blood monocytes or peritoneal cells; and on bone marrow cells including monocytes, myelomonocytic precursors and megakaryocytes.
Tissue sections: PM-2K stains most tissue macrophages in lymphoreticular organs such as thymus, spleen, lymph node and tonsil. It is positive on Kupffer cells of the liver, alveolar macrophages an d macrophages in the interstitial tissues of the kidney, pancreas and many other organs. Proliferating macrophages are positive i n Gaucher's disease , multicentric reticulohistiocytosis and malignant histiocytosis. In MFH infiltrating macrophages are staine d but not tumour cells. Some bone stromal cells but not osteoclast - like multinucleated giant cells are stained in GCT. Microglial cells, osteoclasts and dendritic cells such as Langerhans cells, interdigitating cells and follicular dendritic cells are nega tive.

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