AM32033PU-N Major Vault Protein antibody

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0.1 mg / €310.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Major Vault Protein 1014

AM32033PU-N

Product Description for Major Vault Protein

Mouse anti Human Major Vault Protein 1014.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot.

Properties for Major Vault Protein

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms LRP, Lung-Resistance Related Protein, MVP
Presentation Purified
Reactivity Hu
Applications C, WB
Clonality Monoclonal
Clone 1014
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q14764
Immunogen:
A BALB/c mouse was immunized with 5 μg of affinity purified nuclear extract proteins. Spleen cells were fused with equal number of Sp0Ag-14 myeloma cells.
GeneID:
9961
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Suitable for Western blotting and Immunohistochemistry on Frozen Sections. 
The mAb 1014 can be applied for the detection of MVP/LRP in a large number of eukaryotic cells including the MCF-7 and Hela tumor cell lines. 
The mAb is especially applicable to study the association of Estrogen Receptor with vaults and the study of the mechanism of action of Estrogenic hormones.
Background MVP is identical to lung-resistance related protein (LRP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Amino acid 241-280 of human estrogen receptor (ER), (site of nuclear localization signal sequence), is mapped to be the site of interaction between ER and MVP. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Anti-estrogen 1C1182 shows no effect. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. LRP is a protein overexpressed in many neoplastic tissues and cell lines. Expression of LRP predicts a poor response to chemotherapy.
This 104-kD protein is the major vault protein (MVP) also described as the lung resistance protein (LRP) and has shown to interact with the estrogen receptor. The protein is part of a very large vault ribonucleoprotein complex present in all eukaryotic cells and its structure and protein composition is highly conserved. Because of the size, shape, and protein and RNA composition of this complex the particles are different from other ribonucleoproteins.
Concentration 0.1 mg/ml
General Readings
  1. Abbondanza C, Rossi V, Roscigno A, Gallo L, Belsito A, Piluso G, et al. Interaction of vault particles with estrogen receptor in the MCF-7 breast cancer cell. J Cell Biol. 1998 Jun 15;141(6):1301-10. PubMed PMID: 9628887. (Free PMC Article available, 8 images available)
  2. den Boer ML, Pieters R, Kazemier KM, Rottier MM, Zwaan CM, Kaspers GJ, et al. Relationship between major vault protein/lung resistance protein, multidrug resistance-associated protein, P-glycoprotein expression, and drug resistance in childhood leukemia. Blood. 1998 Mar 15;91(6):2092-8. PubMed PMID: 9490695.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. 
Avoid repeated freezing and thawing. 
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.05% Sodium Azide
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal antibody clone 1014 is specific for a 104-kD protein. This mAb is one of four mAb which recognize different epitopes of the protein.  
Cells whose supernatant showed a positive signal for a 104-kD band were selected and cloned by limited dilution.
Gene ID 9961

Accessory Products

Proteins and/or Positive Controls

Proteins for Major Vault Protein (5 products)

Catalog No. Species Pres. Purity   Source  

Major Vault Protein (transcript variant 2)

Major Vault Protein Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Major Vault Protein (transcript variant 1)

Major Vault Protein Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Major Vault Protein

Major Vault Protein Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Major Vault Protein

Major Vault Protein Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Major Vault Protein

Major Vault Protein Human
  Abnova Taiwan Corp.

Positive controls for Major Vault Protein (1 products)

Catalog No. Species Pres. Purity   Source  

MVP Lysate

Western Blot: MVP Lysate [NBL1-13402] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for MVP.
  Novus Biologicals Inc.
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