AM32041SU-N Major Vault Protein antibody

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1 ml / €430.00
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Quick Overview

Mouse anti Human Major Vault Protein MVP-37

AM32041SU-N

Product Description for Major Vault Protein

Mouse anti Human Major Vault Protein MVP-37.
Presentation: Supernatant
Product is tested for Paraffin Sections, Frozen Sections, Western blot / Immunoblot.

Properties for Major Vault Protein

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms LRP, Lung-Resistance Related Protein, MVP
Presentation Supernatant
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone MVP-37
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q14764
GeneID:
9961
Application Western blotting: 1/50 dilution (chemiluminescence).
Immunohistochemistry: Use ~1/20-1/50 dilution on 4% paraformaldehyde fixed cytospin preparations or frozen tissue sections. 
Pretreatment: 10 min 20 mM Glycine (pH 7.5) and 10 min 6 N Guanidine Hydrochloride in 50 mM Tris-HCl, pH 7.5 (See Schroeijers et al., 2000). 
Immunohistochemistry on Paraffin-Embedded Tissue Sections.
Background MVP is identical to lung-resistance related protein (LRP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Amino acid 241-280 of human estrogen receptor (ER), (site of nuclear localization signal sequence), is mapped to be the site of interaction between ER and MVP. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Anti-estrogen 1C1182 shows no effect. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. LRP is a protein overexpressed in many neoplastic tissues and cell lines. Expression of LRP predicts a poor response to chemotherapy.
This 104-kD protein is the major vault protein (MVP) also described as the lung resistance protein (LRP) and has shown to interact with the estrogen receptor. The protein is part of a very large vault ribonucleoprotein complex present in all eukaryotic cells and its structure and protein composition is highly conserved. Because of the size, shape, and protein and RNA composition of this complex the particles are different from other ribonucleoproteins.
Concentration ~0.25 mg IgG /ml (by ELISA)
General Readings
  1. Schroeijers AB et al., In: Thesis Schroeijers AB, Chapter 4: 2000.
  2. Schroeijers AB, Siva AC, Scheffer GL, de Jong MC, Bolick SC, Dukers DF, et al. The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines. Cancer Res. 2000 Feb 15;60(4):1104-10. PubMed PMID: 10706131.
  3. Kickhoefer VA, Siva AC, Kedersha NL, Inman EM, Ruland C, Streuli M, et al. The 193-kD vault protein, VPARP, is a novel poly(ADP-ribose) polymerase. J Cell Biol. 1999 Sep 6;146(5):917-28. PubMed PMID: 10477748. (Free PMC Article available, 20 images available)
  4. Kickhoefer VA, Rajavel KS, Scheffer GL, Dalton WS, Scheper RJ, Rome LH. Vaults are up-regulated in multidrug-resistant cancer cell lines. J Biol Chem. 1998 Apr 10;273(15):8971-4. PubMed PMID: 9535882.
  5. Scheper RJ, Broxterman HJ, Scheffer GL, Kaaijk P, Dalton WS, van Heijningen TH, et al. Overexpression of a M(r) 110,000 vesicular protein in non-P-glycoprotein-mediated multidrug resistance. Cancer Res. 1993 Apr 1;53(7):1475-9. PubMed PMID: 7680954.
  6. Scheffer GL, Wijngaard PL, Flens MJ, Izquierdo MA, Slovak ML, Pinedo HM, et al. The drug resistance-related protein LRP is the human major vault protein. Nat Med. 1995 Jun;1(6):578-82. PubMed PMID: 7585126.
  7. Izquierdo MA, Scheffer GL, Schroeijers AB, de Jong MC, Scheper RJ. Vault-related resistance to anticancer drugs determined by the expression of the major vault protein LRP. Cytotechnology. 1998 Sep;27(1-3):137-48. doi: 10.1023/A:1008004502861. PubMed PMID: 19002788. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. 
Avoid repeated freezing and thawing. 
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
Stabilizers:
0.7% BSA
State:
Serum Free Culture Supernatant
Supernatant
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal antibody MVP-37 reacts with an internal epitope of MVP/LRP (p110), which is strongly overexpressed in various Human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.
Gene ID 9961

Accessory Products

Proteins and/or Positive Controls

Proteins for Major Vault Protein (5 products)

Catalog No. Species Pres. Purity   Source  

Major Vault Protein (transcript variant 2)

Major Vault Protein Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Major Vault Protein (transcript variant 1)

Major Vault Protein Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Major Vault Protein

Major Vault Protein Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Major Vault Protein

Major Vault Protein Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Major Vault Protein

Major Vault Protein Human
  Abnova Taiwan Corp.

Positive controls for Major Vault Protein (1 products)

Catalog No. Species Pres. Purity   Source  

MVP Lysate

Western Blot: MVP Lysate [NBL1-13402] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for MVP.
  Novus Biologicals Inc.
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